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Short-term toxicity to fish

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Description of key information

In a semi-static acute toxicity test, fish (zebrafish) were exposed for 96 hours according to OECD guideline 203 (1992). The LC-Values based on geometric mean measured test item concentrations were determined to be as follows: LC0 = 1.01 mg/L and LC50 =1.73 mg/L .

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
1.73 mg/L

Additional information

The acute toxicity of the test item to fish (zebrafish) was determined according to OECD No. 203. A 96 hours semi-static test with daily renewal of the test media was performed with the nominal concentrations of 0.25, 0.563, 1.24, 2.73 and 6.00 mg/L (factor: 2.2). The concentrations of the test item were determined via LC-MS/MS from freshly prepared media after 0 and 72 h and from the corresponding 24 h aged test media after 24 and 96. The measured test item concentrations in the freshly prepared media were in the range of 81 to 104 % (0 h) and 94 to 104 % (72 h) of the nominal values. In the corresponding 24 hours aged test media, the measured test item concentrations were in the range of 37 to 76 % (24 h) and 57 to 73 % (96 h) of the nominal values. Therefore, all effect values were given based on the geometric mean measured concentrations of the test item. The validity criteria of the guideline were fulfilled. The LC-Values of the test item (24 - 96 hours) based on geometric mean measured test item concentrations were determined to be as follows: LC0 = 1.01 mg/L, LC50 = 1.73 and LC100 = 5.31 mg/L.