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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: A 2 page study study report. The study was performed pre-GLP. The study was performed according to a method similar to OECD401.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
Very high dose volume. Animals were only observed for signs of intoxication and necropsy was performed on surviving animals.
GLP compliance:
no
Remarks:
pre-GLP
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Tosyl chloride
EC Number:
202-684-8
EC Name:
Tosyl chloride
Cas Number:
98-59-9
Molecular formula:
C7H7ClO2S
IUPAC Name:
4-methylbenzene-1-sulfonyl chloride
Details on test material:
Name: para-toluene sulphochloride
Appearance: white-coloured crystalline powder

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
propylene glycol
Doses:
3.00, 3.60, 4.33, 5.18, 6.20 g/kg
No. of animals per sex per dose:
5
Control animals:
no

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
4 680 mg/kg bw
95% CL:
4 280 - 5 130

Any other information on results incl. tables

Results:

Within a few hours after dosing the rats showed sluggishness. Later on unconsciousness was frequently observed, especially in the two highest dose groups. Death occurred between 3 hours and 3 days after dosing. Thereafter the survivors recovered gradually and looked quite healthy again at the end of the observation period. Macroscopic examination of the survivors revealed no treatment-related gross alterations.

Dose

Mortality

Suspension ml/kg

Test substance g/kg

males

females

%

12.0

3.00

0/5

0/5

0

14.4

3.60

1/5

0/5

10

17.3

4.33

2/5

1/5

30

20.7

5.18

3/5

4/5

70

24.8

6.20

5/5

5/5

100

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Remarks:
Migrated information
Conclusions:
The LD50 of para-toluene sulphochloride was calculated to be 4.68 g per kg body weight with 4.28 and 5.13 as the 95 % confidence limits.
Therefore, the test substance can be classified in Category V according to GHS.
Executive summary:

An acute oral toxicity study was performed according to a method similar to OECD401 and pre-GLP. 5 male and 5 female rats were dosed with 3.00, 3.60, 4.33, 5.18 or 6.20 g/kg bw. The animals were observed for signs of intoxication during a 14-day period, after which autopsies were carried out on the survivors. Within a few hours after dosing the rats showed sluggishness. Later on unconsciousness was frequently observed, especially in the two highest dose groups. Death occurred between 3 hours and 3 days after dosing. Thereafter the survivors recovered gradually and looked quite healthy again at the end of the observation period. Macroscopic examination of the survivors revealed no treatment-related gross alterations.The LD50 of para-toluene sulphochloride was calculated to be 4.68 g per kg body weight with 4.28 and 5.13 as the 95 % confidence limits. Therefore, the test substance can be classified in Category V according to GHS.