Sodium 8-phenylamino-5-(4-(3-sulphonatophenylazo)-1-naphthylazo)naphthalenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

other: read across from similar substance

Adequacy of study:

key study

Study period:

2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: Test No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters."

Deviations:

no

Principles of method if other than guideline:

- Principle of test:
A sample of the tested substance was dissolved in the dilution water at a concentration of 0.1 g/L. The total volume for one dilution is 1000 ml for preliminary as well as for full test. There were used 7 dilution of the test substance.

- Short description of test conditions:
The dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C.
Test duration 96 hours, the temperature of the solutions 22.0 ± 1.0 °C.

- Parameters analysed / observed:
mortality at 48 and 96 h

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: Guppy
- Strain: Poecilia reticulata
- Source: AKVA-SALON, Žižkova 977, 535 01 Přelouč
- Age at study initiation (mean and range, SD): 3 - 4 monts
- Length at study initiation (length definition, mean, range and SD): 2 - 3 cm

ACCLIMATION
- Acclimation period: yes
- Acclimation conditions (same as test or not): same as test

FEEDING DURING TEST
no feeding during test
fasted 72 hours before test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

22.0 ± 1.0 °C

pH:

7.8 (for measured values see attached document with tables)

Dissolved oxygen:

5.40 - 8.6 mg/L O2 (see attached document with tables)

Nominal and measured concentrations:

100.04 mg.L-1

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: open 10 L glass aquarium
- Aeration: without
- No. of organisms per vessel:
Preliminary test: 3 animals
Full test: 10 animals
- No. of vessels per concentration/control (replicates): no replicates

TEST MEDIUM / WATER PARAMETERS
Dilution water: standard dilution water was prepared by mixing 2.5 ml of stock solutions (A,B,C,D) with 1000 ml distilled water
A) 117.6 g CaCl3.2H2O in 1L of distilled water
B) 49.3 g MgSO4.7H2O in 1L of distilled water
C) 25.9 g NaHCO3 in 1L of distilled water
D) 2.3 g KCl in 1L of distilled water
The dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C.


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 48, 96 h

TEST CONCENTRATIONS
- Range finding study: 100, 75, 50, 25 mg.L-1
- Test concentrations: 100, 90, 80, 70, 60, 50, 40 mg.L-1
- Results used to determine the conditions for the definitive study: see attached document with tables

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

80 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

77.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Remarks:

The measured values of pH solution not change more than 1 pH unit.

Executive summary:

The test substance was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 = 80.0 mg.L-1

96 h - LC50 = 77.3 mg.L-1

Description of key information

LC 50 96h = 77,3 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

77.3 mg/L

Additional information

The result of the acute toxicity tests showed some effects to fish.

The species used in the key test was Poecilia Reticulata and the concentration were: 100, 75, 50 and 25 mg/l.

The results of the test were:

- 48h LC50 = 80 mg/l
- 96h LC50 = 77,3 mg/l
The presence of toxic effects for fish is confirmed by the results of the supporting study.

Based on the criteria of the Regulation 1272/2008, the substance needs to be classifies as Aquatic Chronic 3, H412.