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EC number: 222-111-5 | CAS number: 3351-05-1
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
EC50 > 2000 mg/L
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 2 540 mg/L
Additional information
A test was performed on Acid Blue 113 following OECD 221. The results show that the tested item is not toxic to Lemna minor with EC50 > 2540 mg/L. Lemna minor is an aquatic plant that develops his leaves on the surface of the water, while nourishing substances are taken from the water solution. With this tests the observed effect is only related to the potential toxicity of the substance and not to the potential shading effect of a classical Alga study. A deviation to the protocol has been applied to the test recommended for dyes (Michael Cleuvers a, Hans-Toni Ratte, Phytotoxicity of coloured substances: is Lemna Duckweed an alternative to the algal growth inhibition test?Chemosphere 49 (2002) 9–15): "Beakers will be incubated on a black non-reflecting surface. Additionally, the walls of the incubation chambers will also be covered with black fabric in order to avoid reflection".
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