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EC number: 222-111-5 | CAS number: 3351-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- other: read across from similar substance
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Version / remarks:
- Reference from study report: OECD Guideline for Testing of Chemicals, 1981
- GLP compliance:
- yes
- Remarks:
- GLP declaration
- Test type:
- traditional method
- Limit test:
- yes
Test material
- Reference substance name:
- Disodium 6-hydroxy-5-[[4-[[4-(phenylamino)-3-sulphonatophenyl]azo]naphthyl]azo]naphthalene-2-sulphonate
- EC Number:
- 228-412-8
- EC Name:
- Disodium 6-hydroxy-5-[[4-[[4-(phenylamino)-3-sulphonatophenyl]azo]naphthyl]azo]naphthalene-2-sulphonate
- Cas Number:
- 6262-07-3
- Molecular formula:
- C32H23N5O7S2.2Na
- IUPAC Name:
- disodium;(5E)-5-[[4-[(4-anilino-3-sulfonatophenyl)diazenyl]naphthalen-1-yl]hydrazinylidene]-6-oxonaphthalene-2-sulfonate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: VELAZ Praha
- Females (if applicable) nulliparous and non-pregnant: yes
- Microbiological status of animals, when known:
delivered with veterinary attest “QUALITY STATUS” – no parazites, pathogen microorganisms, viruses or mold
- Age at study initiation: 8 weeks
- Weight at study initiation: tested animals; males: 170.8 ± 7.12 g; females: 161.2 ± 4.22 g
- Housing: 5 animals in 1 polypropylene cage T4 type, separated male/female
- Diet (e.g. ad libitum): granulated mixture Altromin 1320 (VELAZ Praha) 12g/animal/day
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 1 week as minimum
ENVIRONMENTAL CONDITIONS
Automatically controlled temperature, humidity and lighting
- Temperature (°C): 22±3
- Humidity (%): 40 – 60%
- Air changes (per hr): not specified
- Photoperiod (hrs dark / hrs light): 12 /12
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- head only
- Vehicle:
- air
- Remark on MMAD/GSD:
- Before starting the exposure a particle size of the initial sample was measured microscopically.
The size of the majority of particles was between 2- 4 µm. - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 6 h
- No. of animals per sex per dose:
- 5 male/5 female
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
immediately after end of exposure, after 1st and 2nd hour, then daily
body weight was measured immediately before exposure, then on the 7th and 14th day
- Necropsy of survivors performed: yes
- Other examinations performed:
clinical signs, body weight, food and water intake, appearance of hair, skin and visible mucous membranes, somatomotor and mental activity, reaction to stimuli, focusing on sensibility and reactivity, lacrimation, functional assessment of the respiratory, circulatory, digestive and urogenital system
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Based on:
- test mat.
- Exp. duration:
- 6 h
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Mortality:
- no
- Clinical signs:
- other: Health condition clinical detection suggests mechanical irritation due to test substance on exposed mucous membranes of the upper respiratory tract, reduced airway patency and insufficient oxygen supply to vital organs.
- Body weight:
- see attached document
- Gross pathology:
- The results of the pathological-anatomical examination revealed a P-deposition of inhaled material particles without local or general response of the organism of animals tested.
Differences depending on the sex of the animals were not recorded. Reactions and autopsy findings were the same.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- No toxic effects observed.
- Executive summary:
A limit test for acute inhalation toxicity with the tested substancewas conducted. The assay was performed according to OECD methodology No. 403. Experimental animals (10 rats Wistar) were exposed to an aerosol inhalation of the pulverized test substance for 4 hours in the inhalation chamber of "head only" type.
During the exposure and even during the 14 day observation period there was no death among tested animals.
Signs of intoxication were clinically determined and give an evidence of mechanical irritation of dust aerosols on exposed mucous membranes of the upper respiratory tract and decreased airway patency and insufficient oxygen supply to vital organs.
Pathological examination had demonstrated a focal infiltration of pulmonary tissues by test substance particles.
Health condition clinical detection suggests mechanical irritation due to test substance on exposed mucous membranes of the upper respiratory tract, reduced airway patency and insufficient oxygen supply to vital organs.
The results of the pathological-anatomical examination revealed a P-deposition of inhaled material particles without local or general response of the organism of animals tested.
Differences depending on the sex of the animals were not recorded. Reactions and autopsy findings were the same.
Limit test results revealed the P-deposition with test substance particles but showed no adverse effects on the health of experimental animals.
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