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Short-term toxicity to fish

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Description of key information

No acute toxicity of the category members of the Long Chain Alcohol Esters to fish up to the limit of water solubility.

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Additional information

Two studies investigated the effects of the category members of the Long Chain Alcohol Esters towards freshwater fish. Testing with the category member fatty acids, C16-18, C12-18-alkyl esters (CAS: 95912-87-1) was performed according to the EU-method C.1 “Acute Toxicity for Fish” (Scholz & Stelter, 1994). A nominal concentration of 10000 mg/L was used for the test. No effects to the zebrafish Brachydanio rerio were observed by the dissolved and even by the undissolved test substance present in the water. The nominal test concentration exceeded the water solubility of the test substance. This resulted in a LC50 of > 10000 mg/L. A further study was performed with the category member decyl oleate (CAS: 3687-46-5) was performed according to the Appendix to the EU-directive 92/69/EWG (1992) (Scholz, 1994). Also in this study, concentrations up to 10000 mg/L were used. At the highest concentration tested 70% mortality occurred after 72 h. However, this was considered to be a physical effect based on the insoluble particles that floated on the top of the water surface. Therefore, the highest test concentration was not taken into account for the determination of the LC50, which was then determined to be > 3000 mg/L. In conclusion, under the conditions of both studies no toxicity to fish was observed up to the limit of the water solubility of the test substances. Based on these findings and the category approach applied it is concluded that the category members of the Long Chain Alcohol Esters are not harmful to fish.

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