3-aminobenzenesulphonic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: screening tests

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well performed study before GLP, with reduced documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: DIN 38412 Part25 for inherent biodegradation

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

other: draft of OECD 301E for ready biodegradation

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-aminobenzenesulphonic acid

EC Number:

204-473-6

EC Name:

3-aminobenzenesulphonic acid

Cas Number:

121-47-1

Molecular formula:

C6H7NO3S

IUPAC Name:

3-aminobenzene-1-sulfonic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

26 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

500 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

48 mg/L

Based on:

test mat.

Details on study design:

Test 1:
in the Zahn-Wellens test biodegradation is determined by measuring the disappearance of DOC or COD. At the time of conducting this test only the COD-method was used. The Zahn-Wellens test isused to test for inherent biodegradability. It specifies that amount of degraded DOC or COD within the test priod of normally 28 days exceeds 70%. But, well biodegradation in the Zahn-Wellens test does not allow an estimation of the "ready biodegradability" according to the OECD Guidelines for testing Chemicals. Substances, which are degraded in the range of 20% to 70% in the Zahn-Wellens test, are classified as primarly or slowly biodegradable.

Test 2:
Test conditions of test 2 were similar to the modified OECD screening test 301E. This method is used to test for ready biodegradability. It specifies that the amount of DOC consumed within 10 days after adaptation (when the 10% threshold is exceeded) and at most 28 days overall must attain 70% of the measured DOC of the test material in the test batches. The special conditions of the OECD screening test according 301E with very low amounts of inoculum simulate surface water conditions, but the amount of inoculum in this test 1976 may have been higher.

Reference substance

Reference substance 1

Reference substance:

diethylene glycol

Remarks:

for test 1

Reference substance 2

Reference substance:

other: D(+)-glucose (monohydrate) for test 2

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

1 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

12

Sampling time:

9 d

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

38

Sampling time:

26 d

% Degradation 4

Parameter:

% degradation (DOC removal)

Value:

42

Sampling time:

32 d

BOD5 / COD results

Results with reference substance:

Test 2 with D(+)-glucose monohydrate: 85% after 23 days
Test 1 with diethylene glycol: 96% after 13 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not inherently but slowly or partly biodegradable

Conclusions:

The test item is not inherently but slowly or partly biodegradable

Executive summary:

A Zahn-Wellens test for inherent biodegradability and a test similar to OECD 301E for ready biodegradability were performed.

For the Zahn-Wellens test no inherent biodegradability was found. In ready biodegradation test, the test item reached only 42% biodegradation (determined by DOC and COD measurements)