Esterification product of castor oil and tetrahydromethyl-1,3-isobenzofuranedione

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-09-05 - 2018-02-24

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

Esterification product of castor oil and tetrahydromethyl-1,3-isobenzofuranedione

EC Number:

700-064-6

Cas Number:

2105830-60-0

Molecular formula:

Not applicable as this is a UVCB substance

IUPAC Name:

Esterification product of castor oil and tetrahydromethyl-1,3-isobenzofuranedione

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The test solutions were prepared as water-soluble fractions (WSFs). 606.1mg of the test substance was added into 6L test water and stirred for 72 hours to prepare a loading rate of 101mg/L ofthe test substance. The vortex depth was about 1/10 of the test solution column. At the end of the stirring period, the mixture was settled for about one hour. Then the raw suspension was withdrawn from the middle of the vessels. Undissolved test substance was removed by filtration over a filter with a pore width of 0 .45µm (discard first 1 OOml).

Test organisms

Test organisms (species):

other: Gobiocypris rarus

Details on test organisms:

The test was performed with Gobiocypris rarus which were bred in Bioassay and Safety Assessment Lab, Shanghai Academy of Public Measurement (ID: GR-SAPM-161209).

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

21.4 - 21.8 °C

pH:

7.48 - 8.02

Dissolved oxygen:

66.3 - 90.2

Nominal and measured concentrations:

Nominal loading rate: 101 mg/L
Measured concentration: below limit of detection (LOD2 = 65.1 ng/mL)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate was tested as a positive control (recently performed on 2017.6.19-6.23). The 96h LCso value of the test substance to Goblocypris rarus (ID: GR-SAPM-161209) was 195mg/L (95% confidence limits: 153-244mg/L).

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LL0

Effect conc.:

> 101 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 101 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No LL50 was calculated since the test was performed as limit test and no toxicity was observed at the loading of 101 mg/L that could be obtained. The results of the limit test were expressed as the loading rate of the test substance (the measured concentrations were below LOD2).

Results with reference substance (positive control):

Potassium dichromate was tested as a positive control (recently performed on 2017.6.19-6.23). The 96h LCso value of the test substance to Goblocypris rarus (ID: GR-SAPM-161209) was 195mg/L (95% confidence limits: 153-244mg/L).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

There was no acute toxicity at the loading of 101mg/L in the limit test. The test results showed that the 96h LL50 for the test substance to Gobiocypris rarus was higher than 101 mg/L (the measured concentration was below LOD2, LOD2=65.1 ng/ml) under the tested conditions. The results of the limit test were expressed as the loading rate of the test substance.

Executive summary:

There was no acute toxicity at the loading of 101mg/L in the limit test. The test results showed that the 96h LL50 for the test substance to Gobiocypris rarus was higher than 101 mg/L (the measured concentration was below the limit of detection LOD2, LOD2=65.1 ng/ml) under the tested conditions. The results of the limit test were expressed as the loading rate of the test substance.