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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro cytogenicity / chromosome aberration study in mammalian cells
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: No GLP data and no guideline was followed. No reference substance was used, there is no information on the number of replicates and the number of observed metaphases was 100 instead of 200 as recommended in the guidelines.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1987

Materials and methods

Principles of method if other than guideline:
A Chinese hamster lung fibroblast cell line (CHL) was used (Ishidate and Odashima, 1977). After a 6-h treatment, the ceils were rinsed with Dulbecco's PBS and recultured with fresh medium for another 18 h. The chromosome preparations were made using the standard air-drying method, and the frequency of the cells with chromosomal aberrations was scored in 100 well-spread metaphases for each concentration group.
GLP compliance:
not specified
Type of assay:
in vitro mammalian chromosome aberration test

Test material

Constituent 1
Chemical structure
Reference substance name:
Chloroacetyl chloride
EC Number:
201-171-6
EC Name:
Chloroacetyl chloride
Cas Number:
79-04-9
Molecular formula:
C2H2Cl2O
IUPAC Name:
chloroacetyl chloride
Details on test material:
- Name of test material (as cited in study report): Chloroacetyl chloride
- Purity: > 99%
- Source: Aldrich Chemical Company. Inc.

Method

Species / strain
Species / strain / cell type:
other: Chinese hamster lung fibroblast cell line (CHL)
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Test concentrations with justification for top dose:
0, 0.06, 0.125, 0.25 and 0.5 mg/ml
Controls
Untreated negative controls:
yes

Results and discussion

Test results
Species / strain:
other: Chinese hamster lung fibroblast cell line (CHL)
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Untreated negative controls validity:
valid
Remarks on result:
other: strain/cell type: Chinese hamster lung fibroblast cell line (CHL)
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

Chloroacetyl chloride showed cytotoxicity at the highest concentration but did not induce chromosomal aberrations.
Executive summary:

A Chinese hamster lung fibroblast cell line (CHL) was used (Ishidate and Odashima, 1977). After a 6-h treatment, the ceils were rinsed with Dulbecco's PBS and recultured with fresh medium for another 18 h. The chromosome preparations were made using the standard air-drying method, and the frequency of the cells with chromosomal aberrations was scored in 100 well-spread metaphases for each concentration group. Chloroacetyl chloride showed cytotoxicity at the highest concentration but did not induce chromosomal aberrations.