Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 206-022-9 | CAS number: 288-88-0
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Sep. 6, 1989 to Dec. 4, 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (OECD 401/423)
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- 1H-1,2,4-triazole CAS No.288-88-0
- Author:
- EPA
- Year:
- 2 009
- Bibliographic source:
- US EPA HPV Challenge Program, Test plan Submission, July 2009
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- 1987 followed
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Version / remarks:
- 1984 followed
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Version / remarks:
- reliability scoring based on 2001
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 1,2,4-triazole
- EC Number:
- 206-022-9
- EC Name:
- 1,2,4-triazole
- Cas Number:
- 288-88-0
- Molecular formula:
- C2H3N3
- IUPAC Name:
- 1H-1,2,4-triazole
- Details on test material:
- - Name of test material (as cited in study report): 1,2,4-TRIAZOL
- Physical state: white solid
- Analytical purity: >98%
- Lot/batch No.: Z81429
- Expiration date of the lot/batch: Aug. 16, 1990
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: BRL, Biological Research Laboratories Ltd. Wölferstrasse 4 CH-4414 Füllinsdorf
- Age at study initiation: males 8 to 9 weeks; females 9 to 11 weeks
- Weight at study initiation: males 179 to 235 g; females 159 to 199 g
- Fasting period before study: 12 to 18 hours
- Housing: Groups of 5 in Makrolon type-3 cages with standard bedding ("Lignocel", Schill AG, CH-4132 Muttenz)
- Diet (e.g. ad libitum): pelleted standard Kliba 343, Batch 55/89 rat maintenance diet ("Kliba" Klingentalmuehle AG, CH-4303 Kaiseraugst) ad libitum
- Water (e.g. ad libitum): community tap water from Itingen ad libitum
- Acclimation period: one week under laboratory conditions after veterinary examination
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3
- Humidity (%): 40 to 70
- Air changes (per hr): 10 to 15
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: bi-distilled water
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 10 mL/kg - Doses:
- 1000, 1500, and 2000 mg/kg
- No. of animals per sex per dose:
- 5 animals/sex/ dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 15 days
- Frequency of observations and weighing: Each animal was examined for changes in appearance and behavior 4 times during day 1 and daily during days 2 to 15. Weighing: Test day 1 (pre-administration), 8, and 15
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights - Statistics:
- LD50 LOGIT-ESTIMATION
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 1 320.39 mg/kg bw
- 95% CL:
- > 1 104.09 - < 1 490.9
- Mortality:
- refer to attached file
- Clinical signs:
- other: refer to attached file
- Gross pathology:
- refer to attached file
Applicant's summary and conclusion
- Interpretation of results:
- Toxicity Category IV
- Remarks:
- Migrated information Harmful if swallowed Criteria used for interpretation of results: other: CLP (EC1272/2008)
- Conclusions:
- According this study, 1,2,4-triazole is classified in category 4 according to the Regulation EC no.1272/2008 (CLP) with a LD50 of 1320 mg/kg bw.
- Executive summary:
A guideline study (OECD 401) was performed in rats to calculate LD50. Rats were exposed to 1,2,4 -triazole at 1000, 1500, and 2000 mg/kg1000, 1500, and 2000 mg/kg bw (a single exposure), and were observed during 15 days. The mortality of each animal was reported and the LD50 was calculated. The LD50 of 1,2,4-triazole is 1320 mg/kg bw in rats.
1,2,4 -triazole is classified in Category 4 according to the regulation EC no.1272/2008 (CLP).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
