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HAT-ISO was tested for aquatic toxicity in a number of tests including EU-Method C.1, Acute toxicity to fish, EU-Method C.2, Acute toxicity to Daphnia and EU-Method C.3, Acute toxicity to algae. Following results were obtained: 

Acute toxicity to fish in the test with Brachydanio rerio revealed a 96 h LD50 of > 250 mg/L (nominal concentration). The 96 h-NOEC in the fish toxicity test was > 250 mg/L (nominal concentration).

Acute toxicity to aquatic invertebrates in the test with Daphnia magna revealed a 48 h EC50 of > 100 mg/L (nominal concentration). The 48 h-NOEC in the Daphnia toxicity test was > 100 mg/L (nominal concentration).

Acute toxicity to freshwater green algae was investigated in two studies. In the key study, toxicity to aquatic algae Desmodesmus subspicatus revealed a 72 h EC50 (growth rate) of > 100 mg/L (nominal concentration). The 72 h-NOEC in the algae inhibition test was > 100 mg/L (nominal concentration). In the supporting study, the 72 h EC50 (growth rate) to Desmodesmus subspicatus was determined to be ca. 76 mg/L (nominal concentration), with a 95 % confidence interval of 54 to 108 mg/L. This value was regarded as invalid, as post testing analysis of the test material revealed that the batch used for testing was contaminated with solvent residues.

The toxicity of HAT-ISO to microorganisms was tested in a 3 h activated sludge respiration inhibition test. The 3 h EC 50 was determined to be > 100 mg/L (nominal concentration). HAT-ISO was considered non-toxic to aquatic microorganisms.

In summary, none of the aquatic toxicity studies revealed toxicity of the test material HAT-ISO. Further testing of HAT-ISO, e.g. in long-term toxicity tests, was considered not scientifically justified.