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Long-term toxicity to fish

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Description of key information

The substance is with high probability not chronically harmful to fish. No long-term effects were observed in a fish early-life stage test with fathead minnow (OECD 210) in the range of water solubility.

Key value for chemical safety assessment

Additional information

The long-term toxicity on fish was investigated with fathead minnow, in a GLP guideline study according to OECD 210 [2017].  Since the test item is poorly soluble, saturated solutions were prepared with a solid-liquid separation systems under consideration of Guidance document on aquatic toxicity testing of difficult items and mixtures, OECD series on testing and assessment number 23, December 14, 2000. The fish early-life stage test was performed in a semi-static system. Loading rates up to 10 mg/L were tested.

Samples for chemical analysis of the actual test concentrations were taken from various intervals during the test of both fresh and old solutions. In addition, stock solutions used for spiking were sampled and analysed. Analysis of the samples taken from the stock solutions showed that these were prepared correctly. The results of analysis of the samples taken from the freshly prepared and spent test solutions showed that it was difficult if not impossible to quantify the test concentrations. Measured concentrations were either extremely low or generally even below the limit of quantification (< 5μg/L). It was concluded that these results could not be used to base test concentration upon. As a consequence, results of the present study were based on the loading rates initially prepared.


The substance did not induce any significant, visible effects on the development of fathead minnow embryos or significantly affect time of hatching or the hatching success at loading rates up to and including 10 mg/L. Further, the substance did not affect survival, growth or development of the larvae at loading rates up to and including 10 mg/L.