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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 Oct. 1990 to 12 Oct. 1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions, e.g. test item concentration and stability not verified
Qualifier:
according to
Guideline:
other: German Industrial Standard test Guideline DIN 38412, part 15
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 7.0 cm (range: 6.5 - 7.8 cm)
- Weight at study initiation: 3.4 g (range: 2.5 - 5.1 g)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day


ACCLIMATION
- Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through system
Water temperature: 19-22°C
Acclimation to test water and test temperature: 3 days
- Type and amount of food: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Feeding frequency: ad libitum.

HOUSING
- Housing water: charcoal filtered dechlorinated water in continuous flow-through
- Aeration: oilfree air
- Light regime: 16 : 8 hours light : dark
- Hardness: 2.5 mM
- Oxygen concentration: > 60 % of maximum saturation
- pH: about 8.0
- Water temperature: 19 - 22 °C
- Duration of housing: about 1.5 month
- Medical treatment: Twice with 0.05 mg/L Malachitegreen chloride and once with 10 mg/L tetracyclinhydrochlorid
- Feeding: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Amount of feed: ad libitum
- Mortality during the last 2 weeks of housing: 0.1%
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
Total hardness: 2.5 mM
Test temperature:
20 - 23 °C
pH:
7.4 - 11.3
Dissolved oxygen:
7.2 - 9.1 mg/L
Nominal and measured concentrations:
Nominal: Control, 100, 215, 464, and 1000 mg/L; additionally a neutralised sample of the test concentration of 1000 mg/L was tested in parallel
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 3.4 g fish /L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 2.2
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Test concentrations: 10, 100, 1000 mg/L
- Results used to determine the conditions for the definitive study: 96-h LC50: >100 - <1000 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 220 - < 460 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: based on not neutralised test solutions
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: based on not neutralised test solutions
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: based on not neutralised test solutions
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
460 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: based on not neutralised test solutions
Details on results:
- Behavioural abnormalities: apathy, narcotic-like state, gasping, tumbling, exopthalmus
- Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: no
- In addition to the test solutions, the highest test concentration of 1000 mg/L was tested in parallel after neutralisation. The mortality was reduced and toxic effects showed with a delay (not neutralised: 100% after 1h; neutralised: 80% after 48 h and 90% after 72 h).

Cumulative mortality

Nominal conc. (mg/L)

Number of fish

Mortality (n; cumulated) after

1 h

4 h

24 h

48 h

72 h

96 h

0

10

0

0

0

0

0

0

100

10

0

0

0

0

0

0

215

10

0

0

2

2

2

2

464

10

0

3

10

10

10

10

1000

10

10

10

10

10

10

10

1000*

10

0

0

0

8

9

9

* = pH adjusted

Symptoms 

Nominal conc. (mg/L)

Symptoms after

1 h

4 h

24 h

48 h

72 h

96 h

0

 

 

 

 

 

 

100

 

 

 

 

 

 

215

 

 

A

A

 

 

464

A

ANT

 

 

 

 

1000

 

 

 

 

 

 

1000*

 

 

ALT

ET

AE

AT

*: pH-adjusted; A: Apathy, N: Narcotic like state, T: Tumbling, L: Gasping, E: Exophthalmos

pH values

Nominal conc. (mg/L)

pH values after

1 h

24 h

48 h

72 h

96 h

0

7.9

7.9

7.7

7.7

7.9

100

10.4

9.2

8.3

8.1

8.1

215

10.7

9.9

9.1

8.6

8.2

464

11.1

10.6

 

 

 

1000

11.3

 

 

 

 

1000*

7.5

7.4

7.4

7.5

7.5

*: pH-adjusted

 

Description of key information

With high probability acutely not harmful to fish.

Key value for chemical safety assessment

Additional information

The toxicity of N,N'-bis(3 -aminopropyl)ethylenediamine (CAS 10563 -26 -5) was studied in a static test acording to national standards (DIN 38412, part 11). The test item concentrations were not analytically verified, but are assumed to be stable regarding the sufficient recovery (> 80%) in the chronic study with Daphnia magna (BASF SE, 2014). The 96 -h LC50 was determined to be > 200 and < 460 mg/L. In addition to the not pH-adjusted test solutions, the highest test concentration of 1000 mg/L was tested in parallel after neutralisation. The mortality was reduced and toxic effects showed with a delay (not neutralised: 100% after 1h; neutralised: 80% after 48 h and 90% after 72 h; BASF AG, 1991; report no. 10F0227/895367).