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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26 June 1989 to 28 June 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Institut National Recherche Chimique Appliqué, France
- Source: originally supplied from 'Institut National Recherche Chimique Appliqué, France' and has been bred from 1978 in the laboratories of BASF
- Age at study initiation (mean and range, SD): 2 to 24 h
- Feeding during test: no
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.7 ± 0.5 mM
Test temperature:
18.85 - 20.85 °C
pH:
0 h: 8.14 to 9.85
48 h: 8.01 to 8.39
Dissolved oxygen:
8.01 to 8.67 mg/L
Nominal and measured concentrations:
Nominal: Control, 6.25, 12.5, 25, 50, 100, 200 mg/L; 200 mg/L (neutralised)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: centrifuge glass tubes with flat bottom; fill volume: 10 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL/animal

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconsituted freshwater (mixture of salts added to deionised water)
- Ca/mg ratio: 4:1
- Conductivity: 600 - 700 µS/cm
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: diffuse

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mobility: 0, 3, 6, 24, 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: not applicable
Duration:
48 d
Dose descriptor:
EC50
Effect conc.:
42.54 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 30.18 to 54.9 mg/L; based on not pH-adjusted test solutions
Duration:
48 d
Dose descriptor:
EC0
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: based on not pH-adjusted test solutions
Duration:
48 d
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: based on not pH-adjusted test solutions
Details on results:
- Mortality of control: 0%
- The highest tested concentration (200 mg/L) was also tested in parallel after pH adjustment. No significant changes were noted compared to the not pH-adjusted test solution.

Cummulative immobility

Nominal conc. (mg/L)

Immobilised animals (n; cumulated)

0 h

3 h

6 h

24 h

48 h

0

0

0

0

0

0

6.25

0

0

0

0

0

12.5

0

0

0

0

0

25

0

0

0

0

0

50

0

0

0

15

20

100

0

0

0

15

20

200

0

0

0

20

20

pH values

Nominal conc. (mg/L)

pH after

0 h

48 h

0

8.14

8.01

6.25

8.39

8.13

12.5

8.64

8.14

25

8.93

8.21

50

9.24

8.32

100

9.59

8.33

200

9.85

8.39

Dissolved oxygen

Nominal conc.

(mg/L)

Dissolved oxygen (mg/L)

0 h

48 h

0

8.11

8.61

6.25

8.06

8.66

12.5

8.02

8.67

25

8.04

8.63

50

8.12

8.60

100

8.02

8.52

200

8.01

8.56

Description of key information

Acutely harmful to aquatic invertebrates

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
42.54 mg/L

Additional information

The acute toxicity of N,N'-bis(3 -aminopropyl)ethylenediamine (CAS 10563 -26 -5) was studied in a static test according to method C2 of Annex V of the EU Directive 79/831/EEC. The test item concentrations were not analytically verified, but are assumed to be stable regarding the sufficient recovery (> 80%) in the chronic study with Daphnia magna (BASF SE, 2014). The 48-h EC50 was determined to be 42.54 mg/L (result based on not pH-adjusted test solutions). In addition to the not pH-adjusted test solutions, the highest test concentration of 200 mg/L was tested in parallel after neutralisation. The toxic effect was similar to the not-adjusted test solution (BASF AG, 1991; report no. 1/0502/2/89 -0502/89).