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Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23 Nov. 2004 to 26 Nov. 2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0 (control), 25 and 100 mg/L sampled at test start and after 72 h of exposure
- Sampling method: chemical analysis of incoulated replicates
- Sample storage conditions before analysis: room temperature, analysed following day (storage < 24 h)
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in test medium for about 10 min at 20 ± 2°C. Test solutions prepared by dilution of stock solution.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none used
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The stock solution was clear.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: green algae
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG (collection of algal cultures, Göttingen, Germany)
- Age of inoculum (at test initiation): seed culture: 4 days ( 23±2 °C; final cell density: 277E4 cells/mL); pre-culture: 3 days ( 23±2 °C; final cell density: 61E4 cells/mL)
- Method of cultivation: algae kept in liquid culture in the Laboratory of Experimental Toxicology and Ecology (BASF, Ludwigshafen, Germany)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
23±2 °C
pH:
7.5 to 8.3
Nominal and measured concentrations:
Nominal (mean measured) concentrations: control, 12.5, 25 (22.5), 50 and 100 (100) mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed with gas permeable silicone sponge caps
- Material, size, headspace, fill volume: Erlenmeyer flasks, 250 mL nominal volume, fill volume 100 mL
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: permanent illumination
- Light intensity and quality: artificial light, universal white (OSRAM L 25), about 60 to 120 µE/(m2*s) at 400 to 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- test parameter: in-vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm)
- Determination of cell concentrations: fluorimeter after 0, 24, 48, 72 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: verified by analytical measurement
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
93.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: verified by analytical measurement
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: verified by analytical measurement
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: verified by analytical measurement
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: verified by analytical measurement

effect values based on biomass:
EbC10 (72 h): 76.3 mg/L
EbC50 (72 h): >100 mg/L
EbC90 (72 h): >100 mg/L
NOEbC (72 h): >/= 100 mg/L
LOEbC (72 h): > 100 mg/L

pH values:

Concentration

[mg/L]

uninoculated

inoculated

0 h

72 h

72 h

Control

8.0

8.3

8.3

12.5

7.9

8.0

8.2

25

7.9

8.0

7.5

50

7.9

8.1

7.6

100

7.9

7.8

7.8

Concentration control analysis:

Nominal concentration

Measured concentration

0 h

72 h

mean

(mg/L)

(mg/L)

(mg/L)

(mg/L)

(% of nominal)

0 (control)

< 10*

< 10*

 -

25

23*

22*

22.5

90

100

101**

99**

100

100

*: based on two measurements

**: based on three measurements

Test results: Calculation of biomass and growth rate and their inhibition after 72 h

Nominal conc.

Biomass after 72 h

Growth rate after 72 h

(mg/L)

(rel. units)

inhibition (%)

(rel. units)

inhibition (%)

0 (control)

178

0.0

0.0590

0.0

12.5

206

-15.5

0.0607

-2.8

25

195

-9.4

0.0582

1.4

50

193

-8.6

0.0580

1.7

100

139

22.0

0.0526

10.9

Description of key information

With high probability acutely not harmful to algae.

Key value for chemical safety assessment

EC10 or NOEC for freshwater algae:
50 mg/L

Additional information

There is one reliable study available regarding the toxic effect of N,N'-bis(3 -aminopropyl)ethylenediamine (CAS 10563 -26 -5). The study was performed according to OECD 201 under GLP conditions. The test species was Desmodesmus subspicatus. The test concentrations were analytically verified (90 to 100% mean recovery). The 72 -h NOErC was determined to be 50 mg/L, the 72 -h ErC50 was > 100 mg/L (BASF AG, 2005; report no. 60E0402/043218).