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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
genetic toxicity in vivo, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: New test system without generally acceptance.

Data source

Reference
Reference Type:
publication
Title:
An in vivo-in vitro replicative DNA synthesis (RDS) test using rat hepatocytes as an early prediction assay for nongenotoxic hepatocarcinogens~ screening of 22 known positives and 25 noncarcinogens
Author:
UnoY, Takasawa H, Miyagawa M, Inoue Y, Murata T and Yoshikawa K
Year:
1994
Bibliographic source:
Mutation Research, 320 (1994) 189-205

Materials and methods

Principles of method if other than guideline:
To evaluate the applicability of an in vivo-in vitro replicative DNA synthesis (RDS) test using rat hepatocytes, we conducted the RDS test with 22 nongenotoxic (Ames-negative) hepatocarcinogens and 25 noncarcinogens (including 3-chloro-p-toluidine, CAS 95-74-9) under standardized conditions and judgement criteria.
GLP compliance:
no
Type of assay:
other: n vivo-in vitro replicative DNA synthesis (RDS) test

Test material

Constituent 1
Chemical structure
Reference substance name:
3-chloro-p-toluidine
EC Number:
202-446-3
EC Name:
3-chloro-p-toluidine
Cas Number:
95-74-9
Molecular formula:
C7H8ClN
IUPAC Name:
3-chloro-4-methylaniline

Test animals

Species:
rat

Results and discussion

Applicant's summary and conclusion

Executive summary:

3-chloro-p-toluidine clearly stimulated RDS at 24 or 39 h after treatment. 3-chloro-p-toluidine is regarded as a noncarcinogen. Therefore the test system is not suitable for mutagenicity testing of 3-chloro-p-toluidine.

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