Vanadium, oxalate complexes

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / micronucleus study

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

no data available

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Reasonably well described study with minor reporting deficiencies: no information on cytotoxicity, no individual results, purity of the test substance not reported.

Data source

Reference

Reference Type:

publication

Title:

Induction of micronuclei in Syrian hamster embryo cells: comparison to results in the SHE cell transformation assay for national toxicology programm test chemicals

Author:

Gibson DP; Brauninger R; Hussain SS; Kerckaert GA; LeBoeuf RA; Isfort RJ; Aardema MJ

Year:

1997

Bibliographic source:

Mutation Research 392: 61-70.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Testing of 16 chemicals in the in vitro micronucleus assay in SHE cells.

GLP compliance:

not specified

Remarks:

publication

Type of assay:

in vitro mammalian cell micronucleus test

Test material

Test material information

Constituent 1

Reference substance name:

Divanadium pentaoxide

EC Number:

215-239-8

EC Name:

Divanadium pentaoxide

Cas Number:

1314-62-1

Molecular formula:

V2O5

IUPAC Name:

divanadium pentaoxide

Details on test material:

- Name of test material (as cited in study report): Vanadium pentoxide
- Physical state: solid
No further details are given.

Method

Target gene:

not applicable

Species / strain

Species / strain / cell type:

mammalian cell line, other: Syrian hamster embryo (SHE) cells

Details on mammalian cell type (if applicable):

For the test, the cells were seeded at 1x10^6 cells/T-25 flask for control, and chemically-treated cultures. Afetr approximately 24 hours, the cells were exposed to the test chemical and cytochalasin B (3 µg/mL in DMSO) for 24 hours.

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

Cytochalasin B (3 µg/mL in DMSO)

Metabolic activation:

without

Metabolic activation system:

not applicable

Test concentrations with justification for top dose:

10, 15, 20, and 25 µg/mL
Dose levels were selected based on solubility and/or toxicity limits.

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

not specified

True negative controls:

no

Positive controls:

yes

Positive control substance:

colchicine

Details on test system and experimental conditions:

METHOD OF APPLICATION: in medium

DURATION
- Exposure duration: 24 hours
- Fixation time (start of exposure up to fixation or harvest of cells): 24 hours and 10 minutes: After determination of the toxicity (see below) remaining cells were suspended in 37°c 0.075M KCl for 5-10 minutes, before the cells were collected by centrifugation and fixed in at least two changes of cold 25:1 methanol/acetic acid.

STAIN (for cytogenetic assays): Cells were stained for 1-5 minutes in a 10% Giemsa solution in Gurr buffer.

NUMBER OF REPLICATIONS: no data

NUMBER OF CELLS EVALUATED: In each treatment group, 500 cells were analysed to determine the percentage of binucleated cells and 1000 binucleated cells were analysed to determine the number of micronucleated cells.

DETERMINATION OF CYTOTOXICITY
- Method: other: number of binucleated cells after 24-hour exposure
After a 24-hour treatment period the media was aspirated off and the cells were collected by trypsinisation. An aliquot of cells was counted to determine the number of live cells (determined by trypan blue exclusion) as a measure of toxicity.

OTHER EXAMINATIONS:
Only cells with distinct cytoplasm and distinct binucleation were analysed for the presence of micronuclei. Only micronuclei that were entirely inside the cytoplasm, separate from the main nucleus, less than approximately one-third the size of the main nuclei, and non-refractile were recorded.

Evaluation criteria:

no data

Statistics:

The number of micronucleated binucleated cells (MNBC) in the treated group was compared to the number of MNBC in the vehicle control group using a one-sided Fisher's exact test where p<0.05 was considered significant.

Results and discussion

Test results

Species / strain:

mammalian cell line, other: SHE cells

Metabolic activation:

without

Genotoxicity:

negative

Remarks:

No statisticaly significant increase in micronucleated binucleated cells (MNBC) was observed.

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Remarks:

(as evidenced by at least a 50% reduction in the relative cell number and/or in the percentage of binucleated cells)

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

True negative controls validity:

not examined

Positive controls validity:

valid

Additional information on results:

No details are reported.

Applicant's summary and conclusion

Conclusions:

Under the test conditions described the test substance, vanadium pentoxide, has no mutagenic potential in the in vitro micronuceus test in Syrian hamster embryo (SHE) cells.

Executive summary:

Vanadium pentoxide as one of 15 other chemical substances was in the in vitro micronucleus assay in SHE at the following concentrations: 10, 15, 20, and 25 µg/mL. Dose levels were selected based on solubility and/or toxicity limits. After a 24-hour treatment period an aliquot of cells was counted to determine the number of live cells (determined by trypan blue exclusion) as a measure of toxicity. Remaining cells were collected by centrifugation, fixed and stained for analysis. In each treatment group, 500 cells were analysed to determine the percentage of binucleated cells and 1000 binucleated cells were analysed to determine the number of micronucleated cells.

Under the test conditions described the test substance, vanadium pentoxide, has no mutagenic potential in the in vitro micronuceus test in Syrian hamster embryo (SHE) cells.