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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin sensitisation

Currently viewing:

Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP and OECD Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 406 (Skin Sensitisation)
Deviations:
no
GLP compliance:
yes
Type of study:
guinea pig maximisation test

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzothiazole-2-thiol
EC Number:
205-736-8
EC Name:
Benzothiazole-2-thiol
Cas Number:
149-30-4
Molecular formula:
C7H5NS2
IUPAC Name:
1,3-benzothiazole-2-thiol
Details on test material:
2-mercaptobenzothiazole, batch no. 01631-077 (supplied by Aldrich Chemie), purity not reported

In vivo test system

Test animals

Species:
guinea pig
Strain:
other: HSD Poc:DH strain
Sex:
female

Study design: in vivo (non-LLNA)

Inductionopen allclose all
Route:
intradermal and epicutaneous
Vehicle:
physiological saline
Concentration / amount:
intradermal induction: 5 % (= 20 mg test item/animal) topical induction: 25 % (= 125 mg test item/animal), challenge: 12 % (= 60 mg test item/animal)
Challengeopen allclose all
Route:
epicutaneous, occlusive
Vehicle:
physiological saline
Concentration / amount:
intradermal induction: 5 % (= 20 mg test item/animal) topical induction: 25 % (= 125 mg test item/animal), challenge: 12 % (= 60 mg test item/animal)
No. of animals per dose:
treatment group: 10, neagitve control: 5

Results and discussion

In vivo (non-LLNA)

Resultsopen allclose all
Reading:
1st reading
Hours after challenge:
48
Group:
test chemical
Dose level:
12 %
No. with + reactions:
7
Total no. in group:
10
Clinical observations:
Appearance and behavior of the test item group were not different from control group
Remarks on result:
other: see Remark
Remarks:
Reading: 1st reading. . Hours after challenge: 48.0. Group: test group. Dose level: 12 %. No with. + reactions: 7.0. Total no. in groups: 10.0. Clinical observations: Appearance and behavior of the test item group were not different from control group.
Reading:
2nd reading
Hours after challenge:
72
Group:
test chemical
Dose level:
12 %
No. with + reactions:
6
Total no. in group:
10
Clinical observations:
Appearance and behavior of the test item group were not different from control group
Remarks on result:
other: see Remark
Remarks:
Reading: 2nd reading. . Hours after challenge: 72.0. Group: test group. Dose level: 12 %. No with. + reactions: 6.0. Total no. in groups: 10.0. Clinical observations: Appearance and behavior of the test item group were not different from control group.
Reading:
1st reading
Hours after challenge:
48
Group:
negative control
Dose level:
0
No. with + reactions:
1
Total no. in group:
5
Clinical observations:
no effects
Remarks on result:
other: Reading: 1st reading. . Hours after challenge: 48.0. Group: negative control. Dose level: 0. No with. + reactions: 1.0. Total no. in groups: 5.0. Clinical observations: no effects.
Reading:
2nd reading
Hours after challenge:
72
Group:
negative control
Dose level:
0
No. with + reactions:
0
Total no. in group:
5
Clinical observations:
no effects
Remarks on result:
other: Reading: 2nd reading. . Hours after challenge: 72.0. Group: negative control. Dose level: 0. No with. + reactions: 0.0. Total no. in groups: 5.0. Clinical observations: no effects.

Any other information on results incl. tables

At the end of the study , the mean body weight of the treatment group animals was slightly higher than that of the control group animals.

The total number of animals with skin reactions was 70 % in the test substance group and 20 % in the control group.

Applicant's summary and conclusion