Pyrochlore, antimony lead yellow

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

mitotic recombination in Saccharomyces cerevisiae

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1979

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The mutagenicity of lead chromate was examined in a battery of microbial tests including: the E. coli PolA+/PolA- survival test, the Salmonella/microsome His+ reversion assay, the E. coli Trp+ reversion test, the E. coli Gal+ forward mutation test, and the S. cerevisiae mitotic recombination assay.

GLP compliance:

not specified

Type of assay:

other: bacterial gene mutation assay; mitotic recombination in Saccharomyces cerevisiae

Test material

Test material information

Constituent 1

Reference substance name:

Lead chromate

EC Number:

231-846-0

EC Name:

Lead chromate

IUPAC Name:

Lead chromate

Results and discussion

Test results

Species / strain:

other: S. typhimurium; E. coli; Saccharomyces cerevisiae

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: Test system: all strains/cell types tested

Any other information on results incl. tables

Lead chromate was positive for mutations in the S. typhimurium His+ reversion assay (at concentrations from 0.2 to 0.4 mg/plate in the presence and absence of S9) and the E. coli Trp+ reversion fluctuation test (at concentrations from 5 uM to 20 uM). Lead chromate was also positive for recombinations in the S. cerevisiae D5 assay at concentrations from 62.5 to 250 ug/ml. Lead chromate had negative responses in the E.coli PolA-/PolA+ assay (at concentrations of 0.2 and 0.3 mg/plate), the E.coli Gal+ forward mutation test (at concentrations from 2.5 to 100 mg/ml), and the E.coli Trp+ reversion plate test (at concentrations of 4 to 60 uM). The authors suggested that the chromate ion is responsible for the observed mutagenicity in Salmonella.

Applicant's summary and conclusion

Conclusions:

Interpretation of results: positive

Executive summary:

The mutagenicity of lead chromate was examined in a battery of microbial tests including: the E. coli PolA+/PolA- survival test, the Salmonella/microsome His+ reversion assay, the E. coli Trp+ reversion test, the E. coli Gal+ forward mutation test, and the S. cerevisiae mitotic recombination assay. Lead chromate was positive for mutations in the S. typhimurium His+ reversion assay (at concentrations from 0.2 to 0.4 mg/plate in the presence and absence of S9) and the E. coli Trp+ reversion fluctuation test (at concentrations from 5 uM to 20 uM). Lead chromate was also positive for recombinations in the S. cerevisiae D5 assay at concentrations from 62.5 to 250 ug/ml. Lead chromate had negative responses in the E.coli PolA-/PolA+ assay (at concentrations of 0.2 and 0.3 mg/plate), the E.coli Gal+ forward mutation test (at concentrations from 2.5 to 100 mg/ml), and the E.coli Trp+ reversion plate test (at concentrations of 4 to 60 uM). The authors suggested that the chromate ion is responsible for the observed mutagenicity in Salmonella.