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Diss Factsheets

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1969
Report date:
1969

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Principles of method if other than guideline:
Method: Before OECD Guideline 404 was established in 1982, skin irritation was tested using an internal method (BASF test). White Vienna rabbits were used. Usually, 2 animals were treated for 1, 5 and 15 minutes and 2 other animals for 20 hours using occlusive conditions. An application site of 2.5 X 2.5 cm was covered with the liquid or powdered and moistened test substance. After the application time, the skin was washed with water which sometimes contained a mild detergent (e.g. Lutrol (50%)). The animals were observed 7 to 8 days and skin changes were recorded on working days. The report usually describes findings after 24 hours and at the end of the observation period.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Isobutyryl chloride
EC Number:
201-194-1
EC Name:
Isobutyryl chloride
Cas Number:
79-30-1
Molecular formula:
C4H7ClO
IUPAC Name:
isobutyryl chloride
Details on test material:
- Name of test material (as cited in study report): Isobuttersäurechlorid

Test animals

Species:
rabbit
Strain:
Vienna White

Test system

Type of coverage:
occlusive
Preparation of test site:
not specified
Vehicle:
unchanged (no vehicle)
Controls:
no
Duration of treatment / exposure:
1, 5 and 15 minutes and 20 hours
Observation period:
8 days
Number of animals:
2 per treatment duration
Details on study design:
TEST SITE
- Area of exposure: 2.5 x 2.5 cm (patch test)

REMOVAL OF TEST SUBSTANCE:
- After the application time, the skin was washed with water.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: erythema score 1 min exposure
Basis:
mean
Time point:
other: 24 h - 8 d
Score:
3
Max. score:
4
Reversibility:
not fully reversible within: 8 days
Remarks on result:
other: after 8 days necrosis with flake off in 1 animal, anaemia in the other
Irritation parameter:
other: edema score 1 min. exposure
Basis:
mean
Time point:
other: 24 h - 8 d
Score:
3
Max. score:
4
Reversibility:
not fully reversible within: 8 days
Remarks on result:
other: after 8 days necrosis with flake off in 1 animal, anaemia in the other
Irritation parameter:
other: effects 5 min exposure
Basis:
mean
Time point:
other: 24 h - 8 d
Reversibility:
not fully reversible within: 8 days
Remarks on result:
other: severe necrosis up to muscle tissue at 24 h; crust formation with bleeding cracks after 8 days
Irritation parameter:
other: effects 15 min. exposure
Basis:
mean
Time point:
other: 24 h - 8 d
Reversibility:
not fully reversible within: 8 days
Remarks on result:
other: severe necrosis up to muscle tissue at 24 h; crust formation with bleeding cracks after 8 days
Irritant / corrosive response data:
Due to the severe skin effects, an application time of 20 hours was not tested.

Applicant's summary and conclusion

Interpretation of results:
highly corrosive
Remarks:
Migrated information