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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1969
Report date:
1969

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Principles of method if other than guideline:
Method: Before OECD Guideline 404 was established in 1982, skin irritation was tested using an internal method (BASF test). White Vienna rabbits were used. Usually, 2 animals were treated for 1, 5 and 15 minutes and 2 other animals for 20 hours using occlusive conditions. An application site of 2.5 X 2.5 cm was covered with the liquid or powdered and moistened test substance. After the application time, the skin was washed with water which sometimes contained a mild detergent (e.g. Lutrol (50%)). The animals were observed 7 to 8 days and skin changes were recorded on working days. The report usually describes findings after 24 hours and at the end of the observation period.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Isobutyryl chloride
EC Number:
201-194-1
EC Name:
Isobutyryl chloride
Cas Number:
79-30-1
Molecular formula:
C4H7ClO
IUPAC Name:
2-methylpropanoyl chloride
Details on test material:
- Name of test material (as cited in study report): Isobuttersäurechlorid

Test animals

Species:
rabbit
Strain:
Vienna White

Test system

Type of coverage:
occlusive
Preparation of test site:
not specified
Vehicle:
unchanged (no vehicle)
Controls:
no
Duration of treatment / exposure:
1, 5 and 15 minutes and 20 hours
Observation period:
8 days
Number of animals:
2 per treatment duration
Details on study design:
TEST SITE
- Area of exposure: 2.5 x 2.5 cm (patch test)

REMOVAL OF TEST SUBSTANCE:
- After the application time, the skin was washed with water.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: erythema score 1 min exposure
Basis:
mean
Time point:
other: 24 h - 8 d
Score:
3
Max. score:
4
Reversibility:
not fully reversible within: 8 days
Remarks on result:
other: after 8 days necrosis with flake off in 1 animal, anaemia in the other
Irritation parameter:
other: edema score 1 min. exposure
Basis:
mean
Time point:
other: 24 h - 8 d
Score:
3
Max. score:
4
Reversibility:
not fully reversible within: 8 days
Remarks on result:
other: after 8 days necrosis with flake off in 1 animal, anaemia in the other
Irritation parameter:
other: effects 5 min exposure
Basis:
mean
Time point:
other: 24 h - 8 d
Reversibility:
not fully reversible within: 8 days
Remarks on result:
other: severe necrosis up to muscle tissue at 24 h; crust formation with bleeding cracks after 8 days
Irritation parameter:
other: effects 15 min. exposure
Basis:
mean
Time point:
other: 24 h - 8 d
Reversibility:
not fully reversible within: 8 days
Remarks on result:
other: severe necrosis up to muscle tissue at 24 h; crust formation with bleeding cracks after 8 days
Irritant / corrosive response data:
Due to the severe skin effects, an application time of 20 hours was not tested.

Applicant's summary and conclusion

Interpretation of results:
highly corrosive
Remarks:
Migrated information