2-methoxyethyl acrylate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given. No details on analytical purity of the test substance. Limited details on inhalation exposure as well as on test animals and environmental conditions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

no details on analytical purity of the test substance; limited details on inhalation exposure as well as on test animals and environmental conditions

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methoxyethyl acrylate

EC Number:

221-499-3

EC Name:

2-methoxyethyl acrylate

Cas Number:

3121-61-7

Molecular formula:

C6H10O3

IUPAC Name:

2-methoxyethyl acrylate

Details on test material:

- Name of test material (as cited in study report): Methyl Cellosolve Acrylate, 2-Methoxyethoxy Propenoate
- Physical state: clear liquid
- Analytical purity: no data
- Other: Sample No. 31-126; Reg. No. 511-01-4353, received from Union Carbide Corporation, Chemicals and Plastics Division, South Charleston, WV

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Remarks on duration:

pre-study: 15 min - 1 h

Concentrations:

pre-study:
1 h: 1576 ppm corresponding to 8.5 mg/L
30 min: 1810 ppm corresponding to 9.8 mg/L
15 min: 1966 ppm corresponding to 10.6 mg/L

main study:
4 h: 1000 ppm corresponding to 5.4 mg/L
4 h: 500 ppm corresponding to 2.7 mg/L
4 h: 250 ppm corresponding to 1.4 mg/L

Dose calculation was based on a molecular weight of 130.14 g/mol and a molar volume of 24.1 L/mol (at 20 °C).

No. of animals per sex per dose:

6

Control animals:

no

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

ca. 2.7 mg/L air

Based on:

test mat.

95% CL:

ca. 1.9 - ca. 3.8

Exp. duration:

4 h

Remarks on result:

other: based on a molecular weight of 130.14 g/mol and a molar volume of 24.1 L/mol (at 20 °C)

Mortality:

pre-study:
1 h (8.5 mg/L): all animals died
30 min (9.8 mg/L): 1 animal died
15 min (10.6 mg/L): no animals died

main study:
4 h (5.4 mg/L): all animals died
4 h (2.7 mg/L): 3 animals died
4 h (1.4 mg/L): no animal died

Clinical signs:

other: Within 30 min exposure to 9.8 mg/L of the test substance, animals showed laboured breathing at 10 min. Animals exposed to 8.5 and 10.6 mg/L did not show clinical signs of toxicity during the 1 h or 15 min exposure period, respectively. After 4 h exposure

Body weight:

All surviving animals showed a weight gain after exposure to the test substance at different concentrations.

Gross pathology:

After exposure to the test substance for 30 min and 1 h, rats dying prior to study termination had bright red livers and gas-filled intestinal tract. Rats surviving to study termination after 15 and 30 min exposure had no remarkable findings.
After 4-h exposure, rats dying prior to scheduled termination had slight haemorrhage of lungs and blood in intestines. For rats surviving until scheduled termination, 2 of 3 rats at 2.7 mg/L had areas of focal consolidation scattered throughout the lungs. All others showed nothing remarkable.

Other findings:

- Other observations:
1 h (8.5 mg/L): irritation of eyes at 10 min; irritation of extremities at 35 min
30 min (9.8 mg/L): irritation of eyes at 5 min; irritation of extremities at 25 min
15 min (10.6 mg/L): irritation of eyes at 5 min; irritation of nose at 10 min

4 h (5.4 mg/L): irritation of eyes at 20 min; irritation of extremities at 25 min
4 h (2.7 mg/L): irritation of eyes at 5 min; irritation of extremities at 1-1/2 h
4 h (1.4 mg/L): irritation of eyes; irritation of extremities at 2-1/2 h

Exposure to 1.4 mg/L or approximately 10% of saturated vapour for a few minutes may cause ocular irritation. A longer exposure to this vapour concentration may cause skin irritation. Experimentation with rats indicates that prolonged exposure to this vapour could lead to irritation of the mucous membranes and skin and pulmonary injury.

Any other information on results incl. tables

Table 1. Acute inhalation toxicity

Exposure time	Dose [mg/L]	Toxicological results*	Observation of clinical signs	Time of death	Mortality (%)
Pre-study
1 h	8.5	6/0/6	---	Days 1 and 2	100
30 min	9.8	1/6/6	at 10 min	Day 1	17
15 min	10.6	0/0/6	---	---	0
Main study
4 h	5.4	6/6/6	at 2 h	Days 1 and 2	100
4 h	2.7	3/6/6	at 4 h	Days 1 and 3	50
4 h	1.4	0/0/6	at 4 h	---	0
LC50 = 2.7 mg/L

*first number = number of dead animals

second number = number of animals with clinical signs

third number = number of animals used

Table 2. Body weight changes

Exposure time	Dose [mg/L]	Weight Change*
Pre-study
1 h	8.5	N/A
30 min	9.8	+++++
15 min	10.6	++++++
Main study
4 h	5.4	N/A
4 h	2.7	+++
4 h	1.4	++++++

*all surviving animals

+ = animals with weight gain

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

CLP: Acute Inhal 3, H331