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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1975
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented study report which meets basic scientific principles. Study was conducted prior to GLP implementation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Principles of method if other than guideline:
test for acute toxicity after parental application
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
o-anisidine
EC Number:
201-963-1
EC Name:
o-anisidine
Cas Number:
90-04-0
Molecular formula:
C7H9NO
IUPAC Name:
2-methoxyaniline
Details on test material:
- Name of test material (as cited in study report): o-Anisidin

Test animals

Species:
cat
Strain:
not specified
Sex:
female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: sodiume chloride
Doses:
10 and 100 mg/kg bw
No. of animals per sex per dose:
2
Control animals:
no
Details on study design:
- Duration of observation period following administration: 24 h
- Necropsy of survivors performed: yes
- Other examinations performed: haematological investigation

Results and discussion

Applicant's summary and conclusion

Conclusions:
Single intraperitoneal application of 100 mg o-Anisidin per kg bw into cats revealed drastic changes in the blood count (Heinz body formation; methaemoglobin formation; increased leucoyte; reduced lymphocyte count) and is fatal within 7 h. 10 mg/kg bw induced only moderate effects on blood parameters tested and was not fatal. Data support conclusion that lethality is caused by MetHb formation.