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EC number: 212-079-0 | CAS number: 760-23-6
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study. Adopted according to OECD SIDS (public available peer reviewed source). The original source is available and has been reviewed.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 996
- Reference Type:
- secondary source
- Title:
- SIDS Initial Assessment Report for 11th SIAM
- Author:
- OECD SIDS
- Year:
- 2 003
- Bibliographic source:
- UNEP Publications
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 3,4-dichlorobut-1-ene
- EC Number:
- 212-079-0
- EC Name:
- 3,4-dichlorobut-1-ene
- Cas Number:
- 760-23-6
- Molecular formula:
- C4H6Cl2
- IUPAC Name:
- 3,4-dichlorobut-1-ene
- Details on test material:
- - Name of test material (as cited in study report): 3,4-dichloro-1-butene
- Purities (identity and concentrations): 99.7%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: males: 133 (125-138) g; females (110-122) g
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3
- Humidity (%): 55 ± 10
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: Sesame oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 0, 6.70 w/v% ; 8.04 w/v%, 9.65 w/v%, 11.58 w/v%, 13.89 w/v%, 16.67% w/v%. - Doses:
- 0 (vehicle), 670, 804, 965, 1158, 1389, 1667 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology - Statistics:
- Probit analysis.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 943 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 828 - 1 068
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 946 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 808 - 1 085
- Mortality:
- Fatalities were found for both sexes at doses of more than 804 mg/kg.
- Clinical signs:
- other: Clinical signs of decreased locomotor activity, deep respiration, ptosis, salivation, flaccidity, adoption of a prone position, piloerection and perinasal soiling with nasal discharge were observed in the treated groups.
- Gross pathology:
- At autopsy, lung enlargement, urine retention and crystalline materials in the urinary bladder, and hemorrhagic black spots in the glandular stomach mucosa were observed in animals.
Any other information on results incl. tables
Table 1. Mortality value of rats treated orally with 3,4 -dichloro-1 -butene in the single dose toxicity test
| Sex | Dose(mg/kg) | Number of animals examined | Mortality |
| Male | 0 | 5 | 0a/5b |
| 670 | 5 | 0/5 | |
| 804 | 5 | 1/5 | |
| 965 | 5 | 2/5 | |
| 1158 | 5 | 5/5 | |
| 1389 | 5 | 5/5 | |
| 1667 | 5 | 5/5 | |
| Female | 0 | 5 | 0/5 |
| 670 | 5 | 0/5 | |
| 804 | 5 | 2/5 | |
| 965 | 5 | 2/5 | |
| 1158 | 5 | 4/5 | |
| 1389 | 5 | 5/5 | |
| 1667 | 5 | 5/5 |
a: number of animals that died; b: number of animals examined.
Based on the test results, 3,4 -dichlorobut-1 -ene is to be classified as Xn R22 according to DSD-DPD, and Acute Tox 4, according to CLP.
Applicant's summary and conclusion
- Executive summary:
MHW, 1996
In an acute oral toxicity study male and Crj:CD(SD) rats were administered orally at doses of 0, 670, 804, 965, 1158, 1389 and 1667 mg/kg (method according to OECD guideline 401) of 3,4 -dichlorobut-1 -ene. The animals were observed for 14 days after administration. Clinical signs of decreased locomotor activity, deep respiration, ptosis, salivation, flaccidity, adoption of a prone position, piloerection and perinasal soiling with nasal discharge were observed in the treated groups. At autopsy, lung enlargement, urine retention and crystalline materials in the urinary bladder and hemorrhagic black spots in the glandular stomach mucosa were observed in animals. Fatalities were found for both sexes at doses of more than 804 mg/kg.
The LD50 values were 943 mg/kg for males and 946 mg/kg for females. Based on the test results, 3,4 -dichlorobut-1-ene is to be classified as Xn R22/20 according to DSD-DPD, and Acute Tox 4, according to CLP (H302, H332, Harmful if inhaled and if swallowed), according to the DSD classification criteria.
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