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EC number: 212-079-0 | CAS number: 760-23-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study. Adopted according to OECD SIDS (public available peer reviewed source). The original source is available and has been reviewed.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Reference Type:
- secondary source
- Title:
- SIDS Initial Assessment Report for 11th SIAM
- Author:
- OECD SIDS
- Year:
- 2 003
- Bibliographic source:
- UNEP Publications
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3,4-dichlorobut-1-ene
- EC Number:
- 212-079-0
- EC Name:
- 3,4-dichlorobut-1-ene
- Cas Number:
- 760-23-6
- Molecular formula:
- C4H6Cl2
- IUPAC Name:
- 3,4-dichlorobut-1-ene
- Details on test material:
- - Name of test material (as cited in study report): 3,4-dichlorbuten-1
- Substance type: product
- Physical state: liquid
- Analytical purity: apporx. 99%
- Batch No.: PE 482
- Stability under test conditions: until 27.07.2002
- Storage condition of test material: at room temperature
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- Himalayan
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: LPT Laboratory of Pharmacology and Toxicology JG, Löhndorf, Post Wankendorf; Germany
- Age at study initiation: approx. 4 to 4.5 months
- Weight at study initiation: 2.0-2.5 kg
- Housing: During the exposure period the animals were kept singly in special restrainers which allowed free movement of the head but prevented a complete body turn. Before and after the 4-hour exposure period the rabbits were kept separately in cages with dimensions of 425 mm x 600 mm x 380 mm.
- Diet (e.g. ad libitum): Altromim 2023 (Altromin GmbH, D-32791 Lage/Lippe, Germany) was served as feed. The feed was available ad libitum before and after the exposure period.
- Water (e.g. ad libitum): The tap water was offered ad libitum before and after the exposure period.
- Acclimation period: 20 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 3
- Humidity (%): 50 ± 20
- Photoperiod (hrs dark / hrs light): 12 / 12
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated skin sites serve as control
- Amount / concentration applied:
- undiluted
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL /patch and animal - Duration of treatment / exposure:
- 4 hour(s)
- Observation period:
- 4 days
- Number of animals:
- 3
- Details on study design:
- TEST SITE
- Area of exposure: approx. 6 cm²
- Type of wrap if used: Gauze Patch which was held in contact with the skin by means of semiocclusive dressing for the duration of the exposure period.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): not reported
SCORING SYSTEM: Draize
EXAMINATION OF THE SKIN: the skin sites were evaluated immediately before the application of the test substance. After 4-hour exposure period the patch was removed and the skin sites were evaluated. Scores were taken 60 minutes, 24, 48, 72 hours and 4 days after patch removal.
Based on most recent guidelines the skin reactions are monitored until the changes observed have completely subsided, however for not more than 14 days after application. Animals that do not reveal any lesions anymore for 24 hours following the first 72 hours of observation are sacrificed.
GENERAL CRITERIA: body weight of all animals was measured at the beginning of the study.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- other: 24, 48, and 72 hours
- Score:
- 0.67
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 hours
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- other: 24, 48, and 72 hours
- Score:
- 1.33
- Max. score:
- 4
- Reversibility:
- fully reversible within: 4 days
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 hours
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- other: 24, 48, and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- other: 24, 48, and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- other: 24, 48, and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritant / corrosive response data:
- Under the test conditions all three rabbits exposed for 4 hours to 0.5 mL 3,4-dichlorobuten-1/patch/animals (semiocclusive condition) showed erythema: animal no. 1 showed 60 minutes to 48 hours after patch removal grade 1erythema ; animal no. 2 showed 60 minutes to 24 hours after patch removal grade 2 erythema and 48 to 72 hours after patch removal grade 1 erythema; animal no. 3: showed 60 minutes to 24 hours after patch removal grade 2 erythema and 48 hours after patch removal grade 1 erythema.
There were no systemic intolerance reactions.
4 days after the treatment all the skin findings were completely gone.
Any other information on results incl. tables
Table 1. : Individual skin examination scores after 4h-exposure to 3, 4-dichlorobut-1 -ene.
Skin effect | Erythema | Edema | ||||||
Scoring | 24h | 48h | 72h | Mean (of 24, 48 and 72 hours in intact skin) | 24h | 48h | 72h | Mean (of 24, 48 and 72 hours in intact skin) |
Animal No. / Sex | ||||||||
1 | 1 | 1 | 0 | 0.67 | 0 | 0 | 0 | 0 |
2 | 2 | 1 | 1 | 1.33 | 0 | 0 | 0 | 0 |
3 | 2 | 1 | 0 | 1.00 | 0 | 0 | 0 | 0 |
Based on the test results the test substance is not to be classified according to DSD-DPD and CLP criteria.
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: other: DSD-DPD and CLP
- Executive summary:
Bayer AG (1999)
In a primary dermal irritation study, the skin irritation/corrosion potential of 3,4-dichlorobut-1-ene was tested. The shaved dorsal skin of the trunk of three male Himalayan rabbits was exposed to 0.5 mL test material for 4 hours under semi-occlusive conditions (OECD guideline 404; EC guideline B.4.). The rabbits were observed for 4 days. Skin reactions were assessed using the Draize scheme approx. 1, 24, 48, 72 hours and 4 days after removal of the test substance.
Under the test conditions all three rabbits exposed for 4 hours to 0.5 mL 3,4-dichlorobuten-1/patch/animals (semiocclusive condition) showed erythema: animal no. 1 showed 60 minutes to 48 hours after patch removal grade 1erythema ; animal no. 2 showed 60 minutes to 24 hours after patch removal grade 2 erythema and 48 to 72 hours after patch removal grade 1 erythema; animal no. 3: showed 60 minutes to 24 hours after patch removal grade 2 erythema and 48 hours after patch removal grade 1 erythema.
There were no systemic intolerance reactions. 4 days after the treatment all the skin findings were completely gone.
On the basis of the present information, it can be concluded that 3,4-dichlorobut-1-ene is not irritating to skin. Therefore the test substance is not to be classified according to DSD-DPD and CLP criteria.
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