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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

Most sensitive fish: Brachydanio rerio
96h-LC50 = 51 mg PBS/L; 16 mg H2O2/L; 5.5 mg B/L

Key value for chemical safety assessment

Additional information

The tests performed by Henkel (1991), Thybaud and Lamy (1996) and Mann (1973) were not considered valid due to insufficient documentation or because the test substance concentrations were not measured during test. The test with zebra fish (Brachydanio rerio), which revealed the lowest NOEC (25 mg PBS/l; 7.8 mg H2O2/l; 2.7 mg B/L) and LC50 (51 mg PBS/L; 16 mg H2O2/L; 5.5 mg B/L) values (Solvay Duphar, 1993), was considered valid without restrictions. Active oxygen concentration was determined before and after the daily renewal of the test solution. At nominal perborate concentrations of 25 - 100 mg/L (corresponding to 7.8 – 31.2 mg H2O2/L) active oxygen concentrations were found to be stable during exposure, which could be attributed to the use of reconstituted water (lacking microbial activity). Only at a nominal concentration of 6.3 mg/l (2 mg H2O2/L) a decline of active oxygen was observed.

No information on the effects of sodium perborate on marine fish species could be identified.

Further information from tests with degradation products

The acute toxicity of hydrogen peroxide has been comprehensively reviewed in the EU Risk Assessment Report (EU Risk Assessment Report on Hydrogen Peroxide). LC50 values were in general lower than 50 mg/l. For Pimephales promelas as the most sensitive species tested in a standardised semistatic test system a 96-hour LC50 value of 16.4 mg/l was observed (measured concentration) which corresponds closely to the 96-hour LC50 value (in hydrogen peroxide equivalents) determined for sodium perborate monohydrate on Brachydanio rerio (see above).

The most sensitive effect value on the acute toxicity of boric acid on fish reviewed by ECB (2009, Assessment Report Boric acid. Product-type 8 (Wood preservative). 20 February 2009) was Catostomus latipinnis with a 96h-LC50 of 125 mg B/L. This value is considerably higher that the LC50 value (in boron equivalents) determined for sodium perborate monohydrate on Brachydanio rerio(see above), demonstrating that the effects of sodium perborate are mainly caused by hydrogen peroxide..