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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented study which meets generally accepted scientific standards. No specification of test substance concentration. Limited documentation.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The substance preparations were injected into the peritoneal cavity of mice. The mice were observed for 14 days and the LD50 was calculated.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Glycerol, ethoxylated
EC Number:
500-075-4
EC Name:
Glycerol, ethoxylated
Cas Number:
31694-55-0
Molecular formula:
(C2 H4 O)n (C2 H4 O)n (C2 H4 O)n C3 H8 O3 3n =>1-<6.5 mol EO
IUPAC Name:
Alkoxylation reaction product of glycerin as starter and ethylene oxyde (EO) as monomer
Details on test material:
- Name of test material (as cited in study report): Schmiermittel LK 2200
- pH 6.5 - 7.5
- Purity: 'technical' purity

Test animals

Species:
mouse
Strain:
other: NMRI Wiga
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: male 28g, females 24g (mean weight)

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Details on exposure:
The doses were administered as a 68.1% (w/v) preparation of the test substance in
aqua dest. as well as undiluted.
Doses:
10000 mg/kg bw
6810 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: Several times on the application day, thereafter once each working day.
- Frequency of weighing: Body weight was determined before the start of the study only, as it was needed for determination of dose.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, gross pathology

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 10 000 mg/kg bw
Mortality:
One male and one female mouse died on day 3 after injection (dose group 10000 mg/kg)
Clinical signs:
After application of the test substance: slight reduction of weight and unspecific symptoms (some animals: convulsion)
Body weight:
No further data
Gross pathology:
nothing abnormal detected
Other findings:
None

Any other information on results incl. tables

# animals which died within
dose (mg / kg bw) concentration # animals 1h 24h 48h 7d 14d
10000 100% 5 male
 5 female
0/5
0/5
0/5
0/5
0/5
0/5
1/5
1/5
1/5
1/5
6810 68,10% 5 male
 5 female
0/5
0/5
0/5
0/5
0/5
0/5
0/5
0/5
0/5
0/5

Applicant's summary and conclusion