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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2003 june 24th to 2003 june 27th
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study performed according to the OECD Guidelines n° 201.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
FC 16
Lot/batch No.: Charge: AF-366
Purity : 99,2 % (according to data of the sponsor)
Water solubility : 0,078 +- 0,041 mg/l (20°C)
Vapor pressure : <= 6,7 x 10-8 Pa (35°C) (according to data of the sponsor)

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
Source: non-axenic strain of the species obtained from 'The Collection of Algal Cultures' of the Institute of Plant Physiology at the University of Göttingen (Germany).
Maintenance of stock cultures : exponentially-growing stock cultures are maintained in the test facility under constant temperature conditions (23 +/- 2°C) at a light intensity in the range 60-120 µE. x m-2 x s-1 (measured in the range 400-700 nm using a spherical quantum flux meter). The nutrient medium (according to BRINGMANN & KHÜN 1977) is renewed once a week. Cell density measurements are using a microcell counter.
Preparation of the pre-cultures : pre-cultures are set up three days before the start of a test. They are grown under identical exposure conditions as the stock cultures, except from the use of a different medium.
test cultures : the algal inocula for a test are taken from an exponentially-growing pre-culture and are mixed with the nutrient medium to make up to a final cell density of about 10 4 cells par millilitre in the test medium.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
21 - 25°C
pH:
8.2 - 10.3
Nominal and measured concentrations:
100 mg/l
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
ca. 70 000 other: cells/ml
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
cell number
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
ca. 200 000 other: cells/ml
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
cell number
Duration:
72 h
Dose descriptor:
EC0
Effect conc.:
ca. 313 333 other: cells/ml
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
cell number
Results with reference substance (positive control):
mean cell density
24 hours : 60000 cells/ml
48 hours : 165556 cells /ml
72 hours : 283889 cells/ml

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No toxic effects against algae at 100 mg/l.
EC0 >= 100 mg/l.