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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
data not available
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
The Acute Mammalian Toxicity of Rare Earth Nitrates and Oxides
Author:
Bruce DW
Year:
1963
Bibliographic source:
Toxicol Appl Pharmac, 5, 750-759

Materials and methods

GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Cerium nitrate, hexahydrate
IUPAC Name:
Cerium nitrate, hexahydrate
Test material form:
not specified
Details on test material:
- Test material identity: Cerium nitrate, as cerium (III) nitrate hexahydrate
- Analytical purity: > 98%
- Impurities: No data
- Lot/batch No: No data
- Expiration date of the lot/batch: No data
- Stability under test conditions: No data
- Storage condition of test material: No data
- Other information: Ce(NO3)3.6H2O were obtained from the Lindsay Chemical Company, West Chicago, Illinois, and the K and K Laboratories, Jamaica, New York.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: no data available
- Age at study initiation: no data available
- Weight at study initiation: 190 g to 250 g
- Fasting period before study: no data available
- Housing: the animals were housed in air-conditioned quarters
- Diet (e.g. ad libitum): yes, ad libitum (food: Rockland rat diet or Rockland mouse pellets)
- Water (e.g. ad libitum): yes, ad libitum
- Acclimation period: no data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data available
- Humidity (%): no data available
- Air changes (per hr): no data available
- Photoperiod (hrs dark / hrs light):no data available

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Details on exposure:
The solution was given in single dose to female rat (50-10 female/groups) at various doses by the intraperitoneal route in volumes equivalent to 0.1% of the total body weight.
Aqueous solutions (pH 4.0-5.9) were prepared immediately before injection.
No. of animals per sex per dose:
5 to 10 animals / groups
Details on study design:
- Duration of observation period following administration: 30 days

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
290 mg/kg bw
95% CL:
238 - 358
Remarks on result:
other: Preliminary experiments indicated no sex difference in the susceptibility of rats to these metals given intraperitoneally.
Mortality:
Although no definite time-mortality pattern was observed, very few of the rats that received lethal doses died during the first 8 days, in contrast to mice.
The majority of animals that failed to survive the 30-day observation period succumbed during the period from 10 to 25 days after
injection.
Gross pathology:
Almost all the animals that died during this period had grossly distended abdomens, and edema of the limbs was observed in many of the animals that received the higher doses. Gross pathologic examination of the animals revealed an inflammatory condition in the peritoneal cavity with massive adhesions and accumulation of hemorrhagic ascitic fluid.

Applicant's summary and conclusion

Conclusions:
An LD50 value of 290 mg/kg was determined in females after intraperitoneal administration of the test substance.