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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vivo mammalian cell study: DNA damage and/or repair
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
The Testicular DNA-Synthesis Inhibition Test (DSI-Test)
Author:
Seiler J.
Year:
1981
Bibliographic source:
In: Stech M. und San R.: Short-Term Tests Chem.Carcinog., Heidelberg

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
- male mice were used
- on the eve of test day: animals received injection of 18,5 kBq (0,5 µCi) (methyl-14C)-thymidine (subcuaneously in the back)
- the next day: prelabeled animals are randomly assigned to test and control groups (receive test substance i.p.)
- 3 hours later: again injection with labeled thymidine subcutaneously (10 µL = 0,37 MBq (10µCi) (methyl-14C)-thymidine
- 45 min later: i.p. injection of 3,0 mg unlabeled thymidine in 0,2 ml physiological saline (provides for a 5x10^4-fold dilution of the radioactive thymidine)
- 30 min later: mice were sacrified and their testes excised
- testes were transferred into 5% trichloroacetic acid (TCA), homogenized and centrifuged
- the pellet was washed twice in 5% TCA, resuspended in fresh 5% TCA, heated for 30 min at 95°C for DNA hydrolysis, cooled and centrifuged
- DNA content was determined photometrically
- radioactivity was determined by liquid scintillation counting
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethylamine
EC Number:
200-834-7
EC Name:
Ethylamine
Cas Number:
75-04-7
Molecular formula:
C2H5NH2
IUPAC Name:
ethanamine
Details on test material:
- Name of test material (as cited in study report): Ethylamine
- Analytical purity: no data

Test animals

Species:
mouse
Strain:
not specified
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Duration of treatment / exposure:
3 hours
Frequency of treatment:
single application
Doses / concentrationsopen allclose all
Dose / conc.:
5 mg/kg bw/day
Dose / conc.:
15 mg/kg bw/day
Dose / conc.:
50 mg/kg bw/day
No. of animals per sex per dose:
no data
Control animals:
yes

Examinations

Statistics:
nonparametric test (Wilcoxon-rank-test or equivalent)

Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Toxicity:
not specified
Vehicle controls validity:
not specified
Negative controls validity:
not specified
Positive controls validity:
not specified

Any other information on results incl. tables

Table 1: DSI Test Results of Ethylamine

Compound

Dose (mg/kg)

%DSI

Ethylamine

5 i.p.

0

15

0

50

5,6

Applicant's summary and conclusion

Conclusions:
Based on the DSI Test results of this publication, the test substance is a Noncarcinogen in mice.
Executive summary:

Male mice were treated with the test substance intraperitoneal. Three dose levels (5, 15, 50 mg/kb bw) were investigated. The results show that the test substance is a Noncarcinogen under the test conditions chosen.