Bis(2-(2-butoxyethoxy)ethoxy)methane

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Bis(2-(2-butoxyethoxy)ethoxy)methane

EC Number:

205-598-9

EC Name:

Bis(2-(2-butoxyethoxy)ethoxy)methane

Cas Number:

143-29-3

Molecular formula:

C17H36O6

IUPAC Name:

5,8,11,13,16,19-hexaoxatricosane

Details on test material:

- Name of test material (as cited in study report): TP-90B Rubber Chemical
- Analytical purity: no data

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Harlan Italy S.r.l., San Pietro al Natisone, Italy
- Age at study initiation: 6-8 weeks
- Weight at study initiation: 201-257 g

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

Area covered: 10% of the total body surface of each animal.

Occlusion: A strip of aluminum foil was placed over the treated site, and the whole assembly held in place by encircling the trunk of the animal with a length of elastic bandage.

Removal of test substance: After a period of 24 hours, the adhesive bandage and gauze dressings were removed. The treated skin was washed gently with warm water to remove residual test item.

Duration of exposure:

24 h

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

not required

Details on study design:

Animals were checked twice daily for mortality and morbidity. Clinical signs were observed immediately upon dosing, approximately 1, 2 and 4 hours after dosing and daily thereafter for a total of 14 days. Body weights were measured on Day -1, immediately prior to dosing (Day 1) and at weekly intervals thereafter (Days 8 and 15). All animals were killed on Day 15 by carbon dioxide narcosis. They were subjected to a gross necropsy examination for both external and internal abnormalities. The cranial, thoracic and abdominal cavities were opened to allow examination of their contents. Larger organs were sectioned. Particular attention was paid to the treated site.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No mortality occurred following dosing in male or female animals.

Clinical signs:

other: No signs of toxicity were observed.

Gross pathology:

No abnormalities were found on necropsy of animals on termination of the study.