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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24 - 26 June 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods
Qualifier:
according to guideline
Guideline:
other: Standard guide for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians (ASTM - 1993)
Qualifier:
according to guideline
Guideline:
other: Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms (US EPA - 1991)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not relevant
Analytical monitoring:
yes
Details on sampling:
During the definitive exposure period, duplicate water samples were removed from each treatment level and the control at 0, 24 and 48 hours
for analysis of substance concentration.
Duplicate samples of the freshly prepared solutions were removed from an additional replicate vessel of each concentration and control
at 0 and 24 hours of exposure.
Duplicate aged solution samples of each concentration and control were removed from a composite of replicates at test termination or
when 100 % mortality was observed.
Each exposure solution sample was collected from the approximate midpoint of the test vessel with a volumetric pipet.
Vehicle:
no
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
Age: <= 24hours
Source: Springborn Laboratories culture facility
Feeding during test: combination of a unicellular green algae (Selenastrum capricornutum YCT mixture)
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
Moderately hard reconstituted water (96 mg/L as CaCO3)
Test temperature:
24 to 26 °C
pH:
7.8
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
Nominal concentrations are based on acute daphnia test results.
Nominal concentrations: 1.7 - 3.3 - 6.5 - 13 - 26 mg/L
Measured concentrations: 1.5 - 2.8 - 5.7 - 12 - 22 mg/L
Control: <0.28 mg/L
Details on test conditions:
TEST SYSTEM
- Material: 40 ml glass vial each containig 40 mL of test solution and sealed with a Teflon lined screw top cap
- Renewal rate of test solution: 24 hours renewal period

OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8 hours of darkness
- Light intensity: 50 to 100 footcandles at the surface of the culture solutions

WATER PARAMETERS
- Source/preparation of dilution water: same source as the water used in the daphnid cultures
- Pesticides: no
- Alkalinity: 68 mg/L
- Conductivity: 370 µmhos/cm
- Total organic carbon: ranged from 0.43 to 0.82 mg/L for the months of december 1993 to may 1994
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
8.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
5.7 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Details on results:
- Mortality (%): 0, 0, 0, 100, 100
- Other effects: lethargic at nominal 13 mg/L
- Mortality of control: 0 %
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no

Validity criteria:
1) In the control not more than 10 per cent of the daphnids have been immobilised;
2) Dissolved oxygen concentration at the end of the test has not been lower than 3 mg/L in control and test vessels.
3) Test substance concentration was sufficiently kept.
Results with reference substance (positive control):
no data
Validity criteria fulfilled:
yes
Conclusions:
48h-LC50 (Ceriodaphnia dubia, immobilisation; semi-static) = 8.2 mg/L (mean measured)
Executive summary:

Mobility inhibition of Ceriodaphnia dubia has been investigated according to U.S. methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms (US EPA - 1991) / U.S. standard guide for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians (ASTM - 1993) and in compliance with GLP.

Applying a semistatic test design (medium change after 24 hr), Ceriodaphnia dubia were exposed for 48 h to an aqueous solution of monobutylamine at nominal concentrations of 1.7 - 3.3 - 6.5 - 13 - 26 mg A.I./L (measured concentrations of 1.5 - 2.8 - 5.7 - 12 - 22 mg A.I./L). The 48h-LC50 was determined at 8.2 mg/L. The No observed acute effects level (NOAEC) is of 5.7 mg/L.

The validity criteria were fulfilled and the study was considered reliable and adequate for the environmental hazard assessment.

Description of key information

48h-LC50 (Ceriodaphnia dubia, immobilisation) = 8.2 mg/L (mean measured concentration, U.S. guideline ASTM - 1991/1993, 1994)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
8.2 mg/L

Additional information

A reliable key study is available investigating mobility inhibition of Ceriodaphnia dubia according to U.S. methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms (US EPA - 1991) / U.S. standard guide for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians (ASTM - 1993) and in compliance with GLP (RL1, 1994).

Ceriodaphnia dubia were exposed (semistatic test design, change of media after 24 h) for 48 h to an aqueous solution of monobutylamine at nominal concentrations of 1.7 - 3.3 - 6.5 - 13 - 26 mg A.I./L (measured concentrations of 1.5 - 2.8 - 5.7 - 12 - 22 mg A.I./L). The 48h-LC50 was determined at 8.2 mg/L (mean measured concentration). The No observed acute effects level (NOAEC) is of 5.7 mg/L.

The validity criteria were fulfilled and the study was considered reliable and adequate for the environmental hazard assessment.