Registration Dossier
Registration Dossier
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EC number: 234-576-1 | CAS number: 12012-35-0
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable study report which meets basic scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- The interaction of cobalt metal with different carbides and other mineral particles on mouse peritoneal macrophages
- Author:
- Lison D. and R.Lauwerys
- Year:
- 1 995
- Bibliographic source:
- Toxic. in vitro 9(3): 341-347
Materials and methods
- Type of study / information:
- In vitro study on the effects on cell integrity
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- In vitro exposure of primary mouse peritoneal macrophages for 18 hours.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Trichromium dicarbide
- EC Number:
- 234-576-1
- EC Name:
- Trichromium dicarbide
- Cas Number:
- 12012-35-0
- Molecular formula:
- Cr3C2
- IUPAC Name:
- {[(chromiomethyl)chromio]methylidyne}chromium
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Chromium carbide Cr3C2. Alfa 24105.
Purity 99.9%.
d50 1.2 µm; 62% < 2 µm
Constituent 1
Results and discussion
Any other information on results incl. tables
Exposure to chromium carbide did not increase the LDH released.
Applicant's summary and conclusion
- Conclusions:
- Chromium carbide did not affect the inegrity of cells, as measured by LDH release.
- Executive summary:
Mouse peritoneal macrophages were cultured for 2 days, after which they were exposed to chromium carbide particles dispersed in Dulbecco's minimum essential medium (DMEM) for 18 hours. At the end of the exposure, lactate dehydrogenase (LDH) was assessed by measuring enzyme activity in the cell-free medium. Exposure with chromium carbide did not increase the release of LDH.
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