N,N'-di-sec-butyl-p-phenylenediamine

Long-term toxicity to aquatic invertebrates

Description of key information

For long term toxicity to Daphnia, test results from hydroquinone, one of the main hydrolysis products of 44PD, were used. Toxicity was investigated in a study conducted according to OECD Guideline 211 (Daphnia magna Reproduction Test) under GLP. Effect parameters observed were inhibition of reproduction, cumulative number of surviving offspring, first day of birth, death and body length of parent daphnias, occurrence of aborted eggs and male individuals. A 21 d NOEC=0.0057 mg HQ/L based on recalculated measured initial concentration was found. After correction for molecular weight, this corresponds to a 21 d NOEC of 0.114 mg 44PD/L.  

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.011 mg/L

Additional information

For 44PD read-across is done from hydroquinone (HQ). The long-term toxicity of hydroquinone was investigated in a study conducted according to OECD Guideline 211 (Daphnia magna Reproduction Test) under GLP. Daphnids were exposed to nominal concentrations of 0 (control), 0.0081, 0.016, 0.033, 0.065, and 0.13 mg HQ/L under semi-static conditions with a 24 hour renewal period of the test water. The test concentrations were monitored via HPLC/UV in three exposure periods on days 0 -1, 10 -11 and 20 -21. The mean values of the measured initial concentrations were 0.0057. 0.012, 0.027, 0.054 and 0.106 mg HQ/L. Effect parameters observed were inhibition of reproduction, cumulative number of surviving offspring, first day of birth, death and body length of parent daphnias, occurrence of aborted eggs and male individuals. A 21 d NOEC=0.0057 mg HQ/L based on recalculated measured initial concentration was found. This corresponds to a 21d NOEC of 0.0114 mg 44PD/L. The testing/validity criteria laid down in the OECD guideline 211 are fulfilled. This study is regarded as reliable without restriction.