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EC number: 247-955-1 | CAS number: 26748-41-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-01-19 to 2001-06-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to
- Guideline:
- EU Method C.6 (Degradation: Chemical Oxygen Demand)
- Version / remarks:
- 1984
- Deviations:
- no
- Qualifier:
- according to
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: WWTP Nieuwgraaf in Duiven, The Netherlands
- Laboratory culture: yes
- Pretreatment: The activated sludge was preconditioned to reduce the endogenous respiration rates.
- Concentration of sludge: 2 mg Dry Weight (DW)/L
- Initial cell/biomass concentration: 2 mg Dry Weight (DW)/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2.1 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD guideline 301
- Test temperature: 19.0 to 21.2 °C
- pH: 7.0 to 7.3 at the start of the test; 6.7 to 7.2 at day 28
- Aeration of dilution water: yes
TEST SYSTEM
- Culturing apparatus: 250 to 300 mL BOD bottles with glass stoppers
- Number of culture flasks/concentration: 10 (containing only inoculum and evaporated silica gel, containg solvent and inoculum, test substance in solvent and inoculum, containing only inoculum) and 6 (containing sodium acetate and inoculum)
- Measuring equipment: electrochemically using an oxygen electrode (WTW Trioxmatic EO 200) and meter (WTW OXI 530)
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes
STATISTICAL METHODS:
The validity of the test is demostrated by an endogenous respiration of 1.6 mg/L at day 28. Furthermore, the differences of the replicate values at day 28 was less than 20%. The biodegradation percentage of the reference compound, sodium acetate, at day 14 was 83. Finally, the validity of the test is shown by oxygen concentrations > 0.5 mg/L in all bottles during the test period. - Reference substance:
- acetic acid, sodium salt
- Test performance:
- The dissolved oxygen concentrations were determined electrochemically using an oxygen electrode and meter. The pH was measured using a Consort pH meter. The temperature was measured and recorded with a thermo couple connected to a data logger.
The dry weight (DW) of the inoculum was determined by filtrating 50 mL of the activated sludge over a preweighed 12 µm Schleier and Schüll filter. - Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 12
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 26
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 53
- Sampling time:
- 140 d
- Remarks on result:
- other: conclusion: inherently biodegradable
- Details on results:
- The test item was biodegraded 26 % at day 28 in the Closed Bottle test. Since, in the prolonged Closed Bottle test the test compound is biodegraded 53 % at day 140, it should be classified as inherently biodegradable. The solvent present in the test substance was biodegraded 44% at day 140 in the Closed Bottle test. Hence, the solvent should be classfified as inherently biodegradable. The test item in solvent was tested in the Closed Bottle in the presence of silica gel. Silica gel was added to permit an accurate administration of the water insoluble test substance. The low water solubility of the test substance may be the reason of the inability to show ready biodegradability of the test substance. Water-insolbule compounds are known for their limited bioavalability. limited bioavailability often results in linear biodegradation curves as found for the solvent.
- Results with reference substance:
- The biodegradation percentage of the reference compound at day 14 was 83.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The test item was biodegraded 26 % at day 28 in the Closed Bottle test. In the prolonged Closed Bottle test this compound was biodegraded 53 % at day 140. The biodegradation reached at the last day of the test demostrates that this test compound should be classified as inherently biodegradable.
- Executive summary:
A ready biodegradability test of tert-butyl peroxyneodecanoate was performed which allows the biodegradability to be measured in an aerobic aqueous medium. The ready biodegradability was determined in the Closed Bottle test performed according to OECD guideline no. 301. The test was prolonged because the pass level was not reached at day 28.
Tert-butyl peroxyneodecanoate caused no reduction in the endogenous respiration. The test substance is therefore considered to be non-inhibitory to the inoculum.
The test item was biodegraded 26 % at day 28 in the Closed Bottle test. In the prolonged Closed Bottle test this compound was biodegraded 53 % at day 140. The biodegradation reached at the last day of the test demostrates that this test compound should be classified as inherently biodegradable.
The test is valid as shown by an endogenous respiration of 1.6 mg/L and by the total mineralization of the reference compound, sodium acetate. Sodium acetate was degraded 83 % of its theoretical oxygen demand after 14 days. Finally, the most important criterion was met by oxygen concentrations > 0.5 mg/L in all bottles during the test period.
Reference
Description of key information
The ready biodegradability of the test item was assessed according to OECD guideline 301 and EU-method C.6. The test item was biodegraded 26 % at day 28 in the Closed Bottle test. In the prolonged Closed Bottle test this compound was biodegraded 53 % at day 140. Therefore it can be concluded that the test item is not ready biodegradable, but inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
A ready biodegradability test of tert-butyl peroxyneodecanoate was performed which allows the biodegradability to be measured in an aerobic aqueous medium. The ready biodegradability was determined in the Closed Bottle test performed according to OECD guideline no. 301. The test was prolonged because the pass level was not reached at day 28. Tert-butyl peroxyneodecanoate caused no reduction in the endogenous respiration. The test substance is therefore considered to be non-inhibitory to the inoculum. The test item was biodegraded 26 % at day 28 in the Closed Bottle test. In the prolonged Closed Bottle test this compound was biodegraded 53 % at day 140. The biodegradation reached at the last day of the test demostrates that this test compound should be classified as inherently biodegradable. The test is valid as shown by an endogenous respiration of 1.6 mg/L and by the total mineralization of the reference compound, sodium acetate. Sodium acetate was degraded 83 % of its theoretical oxygen demand after 14 days. Finally, the most important criterion was met by oxygen concentrations > 0.5 mg/L in all bottles during the test period.
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