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EC number: 247-955-1 | CAS number: 26748-41-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-02-13 to 2012-02-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- adopted 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Version / remarks:
- adopted 1996
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.19, 0.34, 0.62, 1.11 mg/L
- Sampling method: Three replicates samples were taken at the start and two parallel samples were taken at the end of the renewal periods from the test formulation and from the control samples. One sample was taken from the solvent control. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item is a hydrolytically unstable material. In order to minimise hydrolysis before introduction of the fish (treatment) the test solutions were prepared using water-miscible solvent (acetone) as follows: For preparation of each test concentration, individual stock solutions were prepared by dissolving an appropriate amount of test item in acetone. At first step, a work solution (concentration: 20.0 g/L) was prepared by diluting an amount of 0.50 g test item in 25 mL acetone and then the stock solutions were prepared by appropriate diluting of this work solution in acetone. The test solutions of the chosen test concentrations were prepared by appropriate diluting of these stock solutions in the dilution water (ISO medium), each immediately before analytical measurement and treatment.
- Chemical name of vehicle: acetone - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: The test fish were bred and held in Fish Laboratory of TOXI-COOP Zrt. (8237 Tihany, Klebelsberg K. u. 3.).
- Age at study initiation: Juveniles
- Length at study initiation: 2 ± 1 cm
- Method of breeding: similar conditions (water temperature, pH, oxygen concentration etc.) and in the same type of water as that used in the experiment
- Feeding during test: no
ACCLIMATION
- Acclimation period: no acclimatization period - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- approx. 249 mg/L CaCO3
- Test temperature:
- 21.2 – 22.9 °C
- pH:
- 7.45 – 7.97
- Dissolved oxygen:
- 76.8 – 98.1 %
- Nominal and measured concentrations:
- 0.19, 0.34, 0.62, 1.11 and 2.00 mg/L (nominal concentration)
0.07, 0.12, 0.24, 0.46 and 0.73 mg/L (measured concentration) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquariums with 1.5 litre test liquid, covered with glass lids
- Renewal rate of test solution: 24 hours
- No. of organisms per vessel: 7 animals
- No. of vessels per concentration: one replicate
- No. of vessels per control: one replicate
- No. of vessels per vehicle control: one replicate
- Additional test solution control group: An additional test item solution control group (1.1 mg/L) without addition of fish was included in the experiment and analysed at the same occasions as the test concentrations in order to check the possibility of the adsorption of test item on test animals.
- Biomass loading rate: 1.0 mg/L
TEST MEDIUM / WATER PARAMETERS
- in accordance to the guideline
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light: 8 h dark
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.33 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 % conf. limits was not applicable since LC50 was calculated as the geometric mean of the highest test concentration causing no mortality and the lowest concentration causing 100 % mortality
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.24 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.46 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Concentration of the additional test solution control (1.11 mg/L nominal; without addition of fish) was determined at the same occasions as that performed at this concentration including fish. Considerable difference between the two groups was not observed. Therefore, it could be concluded that no adsorption of the test item on fish occurred.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h LC50 value was determined as 0.33 mg/L, and the 96 h NOEC was determined as 0.24 mg/L.
- Executive summary:
The purpose of this study was to evaluate the influence of the test item TBPND on zebrafish (Brachidanio rerio). Young fish were exposed in an acute toxicity test to aqueous test media containing the test item for 96 hours at a range of concentration under semi-static conditions. The frequency of the renewal periods was 24 hours. Based on the results of pre-experiments the following five test concentrations in a geometric series (with a separation factor of 1.8) were tested: 0.19, 0.34, 0.62, 1.11 and 2.00 mg/L (nominal) prepared in solvent (acetone). The measured concentrations deviated more than 20 % from the nominal during the experiment therefore the geometric mean of the measured concentrations were calculated in order to determine exposure concentrations. The calculated geometric mean concentrations were the following: 0.07, 0.12, 0.24, 0.46 and 0.73 mg/L. The 96 h LC50 value was determined as 0.33 mg/L, and the 96 h NOEC was determined as 0.24 mg/L.
Reference
Description of key information
The acute toxicity to fish was assessed according to OECD guideline 203, EU-method C.1 and EPA OPPTS 850.1075. In this 96-hour semi-static acute toxicity test with zebrafish, the LC50 value was 0.33 mg/L (measured concentration). The no-observed-effect concentration (NOEC) was 0.24 mg/L (measured concentration).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.33 mg/L
Additional information
The purpose of this study was to evaluate the influence of the test item TBPND on zebrafish (Brachidanio rerio). Young fish were exposed in an acute toxicity test to aqueous test media containing the test item for 96 hours at a range of concentration under semi-static conditions. The frequency of the renewal periods was 24 hours. Based on the results of pre-experiments the following five test concentrations in a geometric series (with a separation factor of 1.8) were tested: 0.19, 0.34, 0.62, 1.11 and 2.00 mg/L (nominal) prepared in solvent (acetone). The measured concentrations deviated more than 20 % from the nominal during the experiment therefore the geometric mean of the measured concentrations were calculated in order to determine exposure concentrations. The calculated geometric mean concentrations were the following: 0.07, 0.12, 0.24, 0.46 and 0.73 mg/L. The 96 h LC50 value was determined as 0.33 mg/L, and the 96 h NOEC was determined as 0.24 mg/L.
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