Anisaldehyde

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented study report which meets basic scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Principles of method if other than guideline:

Fed. Reg. 38, No. 187, § 1500.42

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Anisaldehyde

EC Number:

204-602-6

EC Name:

Anisaldehyde

Cas Number:

123-11-5

Molecular formula:

C8 H8 O2

IUPAC Name:

4-methoxybenzaldehyde

Details on test material:

- Name of test material (as cited in study report): Anisaldehyd
- Physical state: liquid
- Analytical purity: > 99 % (data from order sheet)

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Gaukler
- Weight at study initiation: male: 3.16 kg; mean of 2 females: 2.46 kg
- Diet (e.g. ad libitum): Ssniff, Intermast

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eye of the same animal

Amount / concentration applied:

0.1 mL

Duration of treatment / exposure:

The substance was not washed out. Single treatment

Observation period (in vivo):

72 h

Number of animals or in vitro replicates:

3

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: not performed

SCORING SYSTEM: according to Draize

TOOL USED TO ASSESS SCORE: fluorescein (only at the final reading)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Remarks:

all animals

Basis:

mean

Time point:

other: 24-48-72 h

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Results 2

Irritation parameter:

iris score

Remarks:

all animals

Basis:

mean

Time point:

other: 24-48-72 h

Score:

0

Max. score:

2

Reversibility:

other: not applicable

Results 3

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

other: 24-48-72 h

Score:

0.33

Max. score:

3

Reversibility:

fully reversible within: 48 h

Results 4

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

other: 24-48-72 h

Score:

0.67

Max. score:

3

Reversibility:

fully reversible within: 72 h

Results 5

Irritation parameter:

conjunctivae score

Basis:

animal #3

Time point:

other: 24-48 -72 h

Score:

0.33

Max. score:

3

Reversibility:

fully reversible within: 48 h

Results 6

Irritation parameter:

chemosis score

Remarks:

all animals

Basis:

mean

Time point:

other: 24-48-72 h

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Any other information on results incl. tables

Animal	Reading	Opacity	Iritis	Redness	Chemosis	Discharge
1	1 h	0	0	2	1	2
2	1 h	0	0	2	1	2
3	1 h	0	0	2	2	2
1	24 h	0	0	1	0	1
2	24 h	0	0	1	0	1
3	24 h	0	0	1	0	1
1	48 h	0	0	0	0	0
2	48 h	0	0	1	0	0
3	48 h	0	0	0	0	0
1	72 h	0	0	0	0	0
2	72 h	0	0	0	0	0
3	72 h	0	0	0	0	0
1	24-48-72 h	0	0	0.33	0
2	24-48-72 h	0	0	0.67	0
3	24-48-72 h	0	0	0.33	0