Registration Dossier

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
other information
Study period:
from 1993-08-19 to 1993-08-25
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: 4e: Documentation insufficient for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
N-methyldioctadecylamine
EC Number:
223-819-7
EC Name:
N-methyldioctadecylamine
Cas Number:
4088-22-6
Molecular formula:
C37H77N
IUPAC Name:
N-methyl-N-octadecyloctadecan-1-amine
Details on test material:
- Trade name material: Genamin SH 301
- Purity: 100%
- Lot/batch No.: 06-HTWC 255

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
no data

Test solutions

Vehicle:
no
Details on test solutions:
no data

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
- Initial biomass concentration: 1600 mg/L
- Source: purification station of Frankfurt/M-Sindlingen

Study design

Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Hardness:
no data
Test temperature:
20°C (+ or - 2°C)
pH:
7.2 (+ or - 2°C)
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
Nominal concentrations: from 500 to 1000 mg/L
Reference substance (positive control):
yes
Remarks:
dichlorophenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L

Applicant's summary and conclusion