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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Link to relevant study record(s)

Description of key information

The substance has been shown to be readily degradable in the closed bottle test (EU Method C.4-E).

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Ready biodegradability was tested according to the Closed Bottle test (EC C.4-E) and in compliance with GLP. A nominal initial test substance concentrations was 3 mg/L. The test was performed with inoculum prepared by filtering the supernatant fraction collected from the secondary effluent of a domestic sewage, which was then aerated until used. The test substance achieved mean degradations of 50% by day 7, and 82% by the end of the study. . The viability of the inoculum and validity of the test were supported by the results of the reference substance, sodium benzoate, which degraded approximately 82% by the end of the test, and achieved 74% biodegradation by day 14. The day-7 results clearly show that the 10-day window criterion was met. The substance is considered to be readily biodegradable under the conditions of the test.