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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Direct Red 239 is not classified as not harmful to aquatic organisms.


This aquatic toxicity assessment was based on the following data:
The short-term toxicity of the structurally related substance "Analogue substance 1" to fish was investigated in three different studies. In a guideline study according to OECD 203 a LC50 (96h) > 100 mg/L was observed on Poecilia reticulate (Clariant 984/c-01, 2002). This result is substantiated by the outcome of two studies conducted by Little et al. in which the TL50 values on Pimephales promelas exceeded 180 mg/L in both cases (Little et al. 1972, 1974). These results are considered to be adaptable for the test substance as well due to their structural similarities. Based on the values obtained for "Analogue substance 1" the test substance is considered to be not acutely not harmful to fish.
The acute toxicity of the test substance on Daphnia magna was investigated in a GLP guideline study according to OECD 202. In this limit test, no toxic effects on the test organisms were observable at an analytically verified concentration of 120 mg/L (BASF SE 50E0861/11X413, 2012). Hence, the test substance is not considered to be acutely harmful to aquatic invertebrates.


A GLP guideline study according to OECD 221 using Lemna gibba as a test organism was conducted (BASF SE 99E0861/11X565, 2012). This test was conducted instead of a study using algae because the test substance is a dyestuff which would influence the photosynthesis capacity of algae cells in the test solution. In this study, no toxic effects on the test organisms were observable at an analytically verified concentration of 120 mg/L. Hence, the test substance is not considered to be acutely harmful to aquatic invertebrates..
Two guideline studies according to OECD 209 are available for the assessment of the toxicity to microorganisms. In a study provided by Clariant (1989) an IC50 value > 100 mg/L was determined while in a study by Kemira (1989) an EC50 > 10000 mg/L was observed. These values are supported by two studies according to OECD 209 using "Analogue substance 1" as test substance. These results are considered to be adaptable for the test substance as well due to their structural similarities. In both studies the EC50 values were determined to exceed 2000 mg/L (Clariant 984/b-01, 2002 and 2791c-07, 2007). Based on these data, the inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.