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EC number: 249-171-5 | CAS number: 28706-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
- Principles of method if other than guideline:
- Estimate of the potential acute hazard after a single intraperitoneal administration of the test material in rats (determination of the LD50).
- GLP compliance:
- no
Test material
- Reference substance name:
- 2 Naphthylamin-(2)-sulfosäure-[(6)-N,N'-Carbonyl-bis-6-aminonaphthol-(1)-sulfonsäure-(3)]
- IUPAC Name:
- 2 Naphthylamin-(2)-sulfosäure-[(6)-N,N'-Carbonyl-bis-6-aminonaphthol-(1)-sulfonsäure-(3)]
- Details on test material:
- - Physical state: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River WIGA, Sulzfeld, FRG
- Age at study initiation: about 12 weeks
- Weight at study initiation: mean males: 200 g; mean females: 180 g
- Fasting period before study: 16 hours
- Housing: in groups of 5 in Type DK-III stainless steel wire mesh cages (supplied by Becker & Co., Castrop-Rauxel, FRG)
- Diet: SSNIFF R (Ssniff Versuchstierdiäten, Soest, FRG) ad libitum
- Water: Fully demineralized water each workday, ad libitum; tap water on public holidays, ad libitum
- Acclimation period: at least one week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 26
- Humidity (%): 45 - 75
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- water
- Details on exposure:
- Single injection into the abdominal cavity. Form of administration: Solution
- Doses:
- 56.2; 82.5; 147.0; 215.0 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Check for moribund and dead animais twice each workday and once daily at weekends and on public holidays. Recording of signs and symptoms < 15', 15', 30', 1 h, 2 h, 4 h, and 5 h after administration of test substance and then once each workday. Weighing of groups before administration, 2nd weighing 2 d, 3rd weighing 4 d, 4th weighing 7 d and 5th weighing 13 d after administration.
- Necropsy of survivors performed: yes
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 115 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 93 - 142
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- ca. 147 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 100 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 70 - 145
- Mortality:
- See table below.
- Clinical signs:
- Clinical signs included dyspnea, apathy, staggering, atonia, trembling, spastic gait, orange/red skin, orange/yellow urine, piloerection, alopecia, exsiccosis, cachexia, ptosis, blood in the urine and poor general state.
- Body weight:
- See table below.
- Gross pathology:
- Animals that died:
147 mg/kg, 82.5 mg/kg: Muscles and adipose tissue: colored by the test substance.
215 mg/kg: Stomach: bloody ulcerations in the glandular stomach; muscles, adipose tissue and subcutis: colored by the test substance.
Sacrificed animals:
56.2 mg/kg, 82.5 mg/kg, 147 mg/kg: Muscles, cartilage and adipose tissue: tinge of the test substance; organs: slightly colored by the test substance.
215 mg/kg: Abdominal organs, cartilage, muscles and adipose tissue: colored by the test substance.
Any other information on results incl. tables
Acute intraperitoneal toxicity:
Dose (mg/kg |
215.0 |
147.0 |
82.5 |
56.2 |
|
Mortality |
|||||
M: Number of animals |
5 |
5 |
5 |
5 |
|
Dead animals after: |
|||||
1 h |
0 |
0 |
0 |
0 |
|
1 d |
0 |
0 |
0 |
0 |
|
2 d |
0 |
0 |
0 |
0 |
|
7 d |
5 |
3 |
0 |
0 |
|
14 d |
5 |
3 |
0 |
0 |
|
|
|||||
F: Number of animals |
5 |
5 |
5 |
5 |
|
Dead animals after: |
|||||
1 h |
0 |
0 |
0 |
0 |
|
1 d |
0 |
0 |
0 |
0 |
|
2 d |
0 |
0 |
0 |
0 |
|
7 d |
5 |
4 |
1 |
0 |
|
14 d |
5 |
4 |
2 |
0 |
|
|
|||||
Mean weight (g) |
|||||
M: Beginning of test: |
210 |
190 |
200 |
200 |
|
After |
2 d |
|
177 |
203 |
208 |
|
4 d |
186 |
|
|
|
|
7 d |
|
164 |
254 |
269 |
|
13 d |
|
241 |
302 |
311 |
F: Beginning of test: |
170 |
190 |
180 |
180 |
|
After |
2 d |
|
183 |
175 |
180 |
|
4 d |
149 |
|
|
|
|
7 d |
|
152 |
178 |
210 |
|
13 d |
|
207 |
211 |
218 |
M = male; F = female; d = day; h = hour
Applicant's summary and conclusion
- Conclusions:
- The LD50 after single injection into the abdominal cavity of male and female rats was determined to be 115 mg/kg bw.
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