4,5-dihydroxy-1,3-dimethylimidazolidin-2-one

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from a laboratory wastewater treatment plants fed with municipal and synthetic sewage
- Concentration of sludge: 30 mg/L

Duration of test (contact time):

28 d

Initial conc.:

20 mg/L

Based on:

DOC

Initial conc.:

109 mg/L

Based on:

test mat.

Details on study design:

TEST CONDITIONS
- Composition of medium: 500 mL destilled water, 13 mL nutrient solution, 5 mL inoculum, 51 mL stock solution test substance, filled up with distilled water to a total volume of 1000 mL.

TEST SYSTEM
- Culturing apparatus: Shimadzu TOC 500
- Number of culture flasks/concentration: 2

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (DOC removal)

Value:

>= 70 - <= 80

Sampling time:

28 d

Interpretation of results:

readily biodegradable, but failing 10-day window

Description of key information

In a study according to OECD guideline 301, the test substance was readily biodegradable, but failed the 10-d window.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable but failing 10-day window

Type of water:

freshwater