4,5-dihydroxy-1,3-dimethylimidazolidin-2-one

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
The stock test solution (293 mg/L based on test substance mass equivalent to 120 mg/L based on the content of main component) was prepared by directly adding 293 mg of test substance to 1L of test medium and stirring for approximately 10 min until visibly dissolved.
The solution was checked visually to ensure that the test substance is completely dissolved.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820
used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Type: open
- Material, size, headspace, fill volume: 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 293 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: 293 mg/L as nominal concentrations based on test substance mass is equivalent to 120 mg/L as nominal concentrations based on the content of “main component” (41%,)

Results with reference substance (positive control):

The EC50(24h) of the reference substance potassium dichromate was 0.70 mg/L (experiment date: 17 Jan 2012, project number: 50E0063/043039).

Since the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.

Validity criteria fulfilled:

yes

Conclusions:

In a Daphnia sp. Acute Immobilisation Test according to OECD guideline 202, the EC50 was >293 mg/L.

Description of key information

EC50 (48 h) > 293 mg/L (nominal, D. magna, OECD 202).

 

293 mg/L as nominal concentrations based on test substance mass is equivalent to 120 mg/L as nominal concentrations based on the content of “main component” (41%)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 293 mg/L