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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
OECD 203, GLP, analytical monitoring.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on test solutions:
Dilution water: purified drinking water
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Danio rerio (Teleostei, Cyprinidae)
- Strain: Danio rerio (Teleostei, Cyprinidae) (HAMILTON-BUCHANAN)
- Source: West Aquarium GmbH, D-37431 Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 2.3 -2.8 cm
- Weight at study initiation (mean and range, SD): mean weight: 0.25 ± 0.2 g
- Method of breeding: Laboratory bred
- Feeding during test: fish were not fed during the test (beginning 24 h prior to initiation)
- Food type: TetraMin® Hauptfutter

- Amount: no data
- Frequency: daily until 24 h before the test

ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): in dilution water of the same quality as used for the test
- Health during acclimation (any mortality observed): no data
Test type:
semi-static
Limit test:
no
Total exposure duration:
96 h
Test temperature:
varied between 21.8 and 22.1 °C
pH:
varied between 7.8 and 8.1
Dissolved oxygen:
in all test vessels between 86% and 100%
Nominal and measured concentrations:
0.74, 1.32, 2.38, 4.29 and 7.72 mg/L (nominal concentrations)
0.59, 0.97, 1.81, 3.28 and 4.45 mg/ (mean measured test concentrations)
The measured values deviated by more than 20 % from nominal values at all dose levels.
Details on test conditions:
Dilution water was prepared according to the test guideline (OECD 203) following internal SOP’s.

Test concentrations:
Aliquots of the stock solution were shaken for 24 h, then they were diluted with purified drinking water (dilution water).


Test priciples and procedure:

10 fish were used for each test concentration including controls.
Only fish in good health and free from any apparent malformation were used.


15-l glass aquaria were used as test vessels and filled with 10 l of test solution. The test animals were added to the previously prepared test solution; controls were kept in dilution water (blank).

The aquaria were slightly aerated via a glass capillary throughout the test.


The fish were exposed to various concentrations of the test substance for a period of 96 hours under semi static conditions. The test medium was changed completely every day. Visible abnormalities observed during the first 6 hours of the experiment were recorded. Mortalities and visual abnormalities were recorded at 24 h, 48 h, 72 h, and 96 h.

The test temperature was 21.8° C to 22.1 ° C. The pH of the dilution water was 7.8 to 8.1. The oxygen saturation in all test vessels was between 86 % and 100 %.
The light/dark rhythm was 14h/10h.

The pH, temperature and the oxygen concentrations of the control and of all the test concentrations were measured daily.
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.79 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 1.58 - 2.04 (95% confidence limits of LC50)
Duration:
96 h
Dose descriptor:
LC10
Effect conc.:
1.45 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
0.97 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: The effect concentrations refer to the mean measured concentrations, because the measured concentrations deviated by more than 20 % from the nominal values.
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
1.81 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: The effect concentrations refer to the mean measured concentrations, because the measured concentrations deviated by more than 20 % from the nominal values.
Details on results:
The measured values deviated by more than 20 % from nominal values at all dose levels. Thus, the mean measured concentrations were taken as basis for the calculation of the lethal effect concentrations.
Sublethal observations / clinical signs:

 Test concentrations - nominal and measured concentrations of the test substance
                                                                                     Measured test concentrations
Nominal test concentrations [mg/L]  0h  24h*  48h  72h  96h  Mean ± s.d.  Deviations [%] from nominal values
 control  0.00  0.00/0.00  0.00  0.00  0.00   0.00 ± 0.00  
 0.74 0.58  0.44/0.66  0.65  0.67   0.55   0.59 ± 0.09  - 20.2
 1.32 0.89  0.67/1.11  1.11   1.16   0.91  0.97 ± 0.18  - 26.4
 2.38 1.58  1.19/2.39   1.91   2.04  1.76   1.81 ± 0.39  -24.0
 4.29 2.97  2.28/3.93   3.92(old)   n.d.   n.d.   3.28 ± 0.74  -23.7
 7.72 4.71  4.19/n.d.  n.d.   n.d.   n.d.   4.45 ± 0.30  -42.4

 (*) test concentration before renewal of the test solution/ freshly prepared test solution;

s.d. = standard deviation;

n.d. = not determined (all fish dead)

 Cumulative Mortality of Danio rerio (Zebra Fish) (a)
                                                                                     Cumulative mortality of fish
 Nr   Test concentration (mg/l)  24h  48h  72h  96h
 0/0  blanc  0  0   0   0 
 1 0.59  0  0   0   0 
 2 0.97  0  0   0   0 
 3 1.81  0   0   4  5 
 4 3.28  1   10   10   10 
 5 4.45  10  10   10   10 

 (a) In all test concentrations the fish were inconspicuous during the exposure time of 96h 

Validity criteria fulfilled:
yes
Remarks:
-Criteria of OECD 203: Mortality of controls <1
Conclusions:
In a valid guideline study (OECD 203, semi-static conditions), the 96 hr LC50 for Danio rerio (Teleostei, Cyprinidae) is 1.79 mg/L .
Executive summary:

In a 96-h acute toxicity study, Danio rerio (Teleostei, Cyprinidae) were exposed to Isobornyl methacrylate

at 0 (control), 0.74, 1.32, 2.38, 4.29, 7.72 mg/L nominal and 0 (control), 0.59, 0.97, 1.81, 3.28, 4.45 mg/L measured concentrations under semi-static conditions. The 96-h LC50was 1.79 (1.58 - 2.04 95 % confidence limits) mg a.i./L. The LC10and NOEC values, based on mortality effects, were 1.45 mg/L and 0.97 mg a.i./L, respectively. The effect concentrations refer to the mean measured concentrations, because the measured concentrations deviated by more than 20 % from the nominal values. Based on the results of this study, Isobornyl methacrylate would be classified as toxic to Danio rerio (fish) in accordance with the classification system of the EU.

 

This toxicity study is classified as acceptable and satisfies the guideline requirement for OECD 203, Danio rerio (Zebra fish) toxicity study.

 

Results Synopsis

 

Test organism size/age: mean weight: 0.25 ± 0.2 g, length: 2.3 -2.8 cm

Test Type: semi-static, change of test medium: completely every 24 hours

 

LC50: 1.79 mg a.i./L                         95% C.I.: 1.58 to 2.04 mg a.i./L

NOEL: 0.97 mg a.i./L                      

LC10:  1.45 mg a.i./L}                      

Endpoint(s) Effected: mortality

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

One reliable study for Isobornyl methacrylate on freshwater fish acc. OECD 203

is available. LC50 (96h) on Danio rerio was determined to be 1.79 mg/L

 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1.79 mg/L

Additional information