1-methylpiperazine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 - 15 November 1967

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 401.
A test group consisting of 5 animals/sex was treated by single gavage application with an aqueous solution of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 7 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy.

GLP compliance:

no

Remarks:

pre-GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-methylpiperazine

EC Number:

203-639-5

EC Name:

1-methylpiperazine

Cas Number:

109-01-3

Molecular formula:

C5H12N2

IUPAC Name:

1-methylpiperazine

Details on test material:

- Name of test material (as cited in study report): Monomethylpiperazin
- Analytical purity: 99 %

Specific details on test material used for the study:

- Name of test material (as cited in study report): Monomethylpiperazin
- Analytical purity: 99 %

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 118 - 236 g

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

800, 1600, 2000, 2500, 3200, 4000 cmm/kg bw (722, 1444, 1806, 2258, 2890, 3612 mg/kg bw - conversation in mg/kg is based on the density: d= 0.903 g/cm3).

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 2 258 mg/kg bw

Remarks on result:

other: conversation in mg/kg is based on the density: d=0.903 g/cm3.

Mortality:

3612 mg/kg: all animals died within 24 h.
2890 mg/kg: 1 male and 5 females died within 24 h.
2258 mg/kg: 1 male and 5 females died within 24 h.
Lower dose levels: no mortalities.

Clinical signs:

other: 3612 mg/kg: 3 h post application abdominal and lateral position, closed eyes, irregular respiration. 24 h post application ruffled fur, apathy, closed eyes, shallow respiration. 2890 mg/kg: After 30 min abdominal position, intermittent respiration, closed

Gross pathology:

Animals that died: All animals showed stomach ectasia with liquid-mucous, partially bloody content. 10 x blood filled secretion vesicles in the nose.

Any other information on results incl. tables

Mortality:

Dose (mg/kg)	Gender	Conc.(%)	dead within 1h	dead within 24 h	dead within 48 h	dead within 7 days
3612	male	30	0/5	5/5	5/5	5/5
3612	female	30	0/5	5/5	5/5	5/5
2890	male	30	0/5	1/5	1/5	1/5
2890	female	30	0/5	5/5	5/5	5/5
2258	male	20	0/5	1/5	1/5	1/5
2258	female	20	0/5	5/5	5/5	5/5
1806	male	20	0/5	0/5	0/5	0/5
1806	female	20	0/5	0/5	0/5	0/5
1444	male	20	0/5	0/5	0/5	0/5
1444	female	20	0/5	0/5	0/5	0/5
722	male	8	0/5	0/5	0/5	0/5
722	female	8	0/5	0/5	0/5	0/5

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

Based on the results of this study, the acute oral LD50 was estimated to be ca. 2258 mg/kg bw.