1-methylpiperazine

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08. Nov 1967 - 16. Nov 1967

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Principles of method if other than guideline:

Method: BASF-Test. 50 µL of the test substance were applied to the conjunctival sac of one eye in 2 animals. The animals were observed after 10 min, 1 and 24 h on the day of treatment and on day 3 and 8 afterwards. The eyes were not washed out after 24 hours as specified in OECD Guideline 405.

GLP compliance:

not specified

Test material

Test material information

1

Reference substance name:

1-methylpiperazine

EC Number:

203-639-5

EC Name:

1-methylpiperazine

Cas Number:

109-01-3

Molecular formula:

C5H12N2

IUPAC Name:

1-methylpiperazine

Details on test material:

- Name of test material (as cited in study report): Monomethylpiperazine
- Analytical purity: 99 %

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.63 and 2.68 kg

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: The adjacent eye served as saline control.

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 µl

Duration of treatment / exposure:

single application

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

24 h

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 2

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

24 h

Score:

2

Max. score:

3

Reversibility:

not fully reversible within: 8 days

Results 3

Irritation parameter:

chemosis score

Basis:

animal #1

Time point:

24 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 4

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

24 h

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 5

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

24 h

Score:

0

Max. score:

4

Reversibility:

fully reversible

Results 6

Irritation parameter:

chemosis score

Basis:

animal #2

Time point:

24 h

Score:

0

Max. score:

4

Reversibility:

fully reversible

Results 7

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

48 h

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 8

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

48 h

Score:

2

Max. score:

4

Reversibility:

fully reversible

Results 9

Irritation parameter:

chemosis score

Basis:

animal #1

Time point:

48 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: within 8 days

Results 10

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

48 h

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 11

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

48 h

Score:

0

Max. score:

4

Reversibility:

fully reversible

Results 12

Irritation parameter:

chemosis score

Basis:

animal #2

Time point:

48 h

Score:

0

Max. score:

4

Reversibility:

fully reversible

Results 13

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

72 h

Remarks on result:

not measured/tested

Results 14

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

72 h

Remarks on result:

not measured/tested

Results 15

Irritation parameter:

chemosis score

Basis:

animal #1

Time point:

72 h

Remarks on result:

not measured/tested

Results 16

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

72 h

Remarks on result:

not measured/tested

Results 17

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

72 h

Remarks on result:

not measured/tested

Results 18

Irritation parameter:

chemosis score

Basis:

animal #2

Time point:

72 h

Remarks on result:

not measured/tested

Results 19

Irritation parameter:

iris score

Basis:

animal #1

Time point:

24/48/72 h

Remarks on result:

not measured/tested

Results 20

Irritation parameter:

iris score

Basis:

animal #2

Time point:

24/48/72 h

Remarks on result:

not measured/tested

Any other information on results incl. tables

Findings: animal1/animal2

Time	corneal opacity	erythema	chemosis
1 h	3/3	0/0	0/0
24 h	3/3	2/0	2/0
48 h	3/3	2/0	2/0
72 h	-/-	-/-	-/-
8 d	3/3	2/0	0/2

The original BASF grading was converted into the numerical grading according the OECD Draize system.

The 72 h reading is missing.

Mean values over 24 and 48 h (the 72 h reading is missing).

Animal1: Erythema: 2 ; Opacity: 3; Chemosis: 2;

Animal2: Erythema: 0 ; Opacity: 3; Chemosis: 0;

At the end of the observation period after 8 days the eyes were covered with purulence and crust formation was found in the periphery. In one animal after 5 days staphyloma formation was induced. The latter and opacity grade 3 are considered to be irreversible findings.