Menthol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990-03-19 till 1990-04-17

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: UBA-Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, LC0, LC50, LC100; 48-96 h" (Mai 1984)

Version / remarks:

Guideline proposal of the German Federal Environmental Agency (UBA)

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 131136

Analytical monitoring:

yes

Details on sampling:

direct weighted

Vehicle:

no

Details on test solutions:

test medium (synthetic origin, prepared according to ISO)

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- origin: West-Aquarium (Bad Lauterberg, Germany)
- ventilated test system
- loading rate: 10 animals/vessel (3 month old)
- Length: 2.5 to 3.5 cm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

22.3°C

pH:

7.6

Dissolved oxygen:

8.5 mg/L
99.6 %

Nominal and measured concentrations:

Nominal: 7.8, 11, 16, 22 and 31 mg/L
Measured (in mean): 6.4, 9.3, 11.3, 18.7 and 26.2 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

22.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

31 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The fish were exposed to 5 nominal test substance concentrations in the range of 7.8 to 31 mg/L. In all test media, undissolved substance particles
remained on the water surface. Analytical measurements revealed that the test substance concentrations decreased below 80 % of the nominal within 96 h, probably due to volatilization.

Validity criteria fulfilled:

yes

Remarks:

- dissolved oxygen concentration remained above 60% of the air-saturation value throughout the exposure period. - No mortality in the controls by the end of the test.

Conclusions:

In a study with fish (Danio rerio) a LC50 of 22.3 mg/L (geom. mean from nominal concentration) after 96 hours is obtained.

Executive summary:

The study was conducted according to Umweltbundesamt (UBA) -Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, Mai 1984" with fish (Danio rerio). The fish were exposed to 5 nominal concentrations of DL-Menthol in the range of 7.8 to 31 mg/L ( 7.8, 11, 16, 22 and 31 mg/L). 10 fish were tested per concentration level. A LC50 of 22.3 mg/L (geom. mean from nominal concentration) after 96 hours is obtained.

Description of key information

The study was conducted according to Umweltbundesamt (UBA) -Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, Mai 1984" with fish (Danio rerio). The fishs were exposed to 5 nominal concentrations of DL-Menthol in the range of 7.8 to 31 mg/L ( 7.8, 11, 16, 22 and 31 mg/L). 10 fish were tested per concentration level. A LC50 of 22.3 mg/L (geom. mean from nominal concentration) after 96 hours is obtained.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

22.3 mg/L