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The test item Leuco Sulfur Black 1 was investigated for its ready biodegradability in a manometric respirometry test over 28 days according to the EU Commission Directive 92/69 EEC, C.4-D Commission Regulation (EC) No 440/2008, C.4-D and the OECD Guideline for Testing of Chemicals No. 301 F.

The biochemical oxygen demand (BOD) of the test item Leuco Sulfur Black 1 in the test media was in the normal range found for the inoculum controls.

Consequently, Leuco Sulfur Black 1 was not biodegradable under the test conditions within 28 days.

In the toxicity control, containing both Leuco Sulfur Black 1 and the reference item sodium benzoate, Leuco Sulfur Black 1 had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 142 mg/L.

In the procedure controls, the reference item sodium benzoate was degraded by an average of 80% by Exposure Day 14, and reached an average biodegradation of 82% by the end of the test (Day 28), thus confirming suitability of the activated sludge.