Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N''-hexane-1,6-diylbis[N'-benzylurea]
EC Number:
254-274-3
EC Name:
N,N''-hexane-1,6-diylbis[N'-benzylurea]
Cas Number:
39072-70-3
Molecular formula:
C22H30N4O2
IUPAC Name:
3-benzyl-1-{6-[(benzylcarbamoyl)amino]hexyl}urea
Details on test material:
Description White powder (determined at NOTOX)
Batch 259-635
Purity > 90%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Frequency at t=0 h, t=24 h and t=96 h
Volume 2 ml from the approximate centre of the test vessels
Storage Samples were stored in a freezer until analysis

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Mean length 3.4 ± 0.1 cm
Mean weight 0.70 ± 0.10 g

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
180 mg CaCO3 per litre
Test temperature:
21.0 - 22.3°C
pH:
pH of 7.5 - 9.4

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
The 96h-LC50 exceeded 12 µg/l, being the mean measured concentration in a 0.45 µm filtered solution prepared at a loading rate of 100 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes