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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N''-hexane-1,6-diylbis[N'-benzylurea]
EC Number:
254-274-3
EC Name:
N,N''-hexane-1,6-diylbis[N'-benzylurea]
Cas Number:
39072-70-3
Molecular formula:
C22H30N4O2
IUPAC Name:
3-benzyl-1-{6-[(benzylcarbamoyl)amino]hexyl}urea
Details on test material:
Description White powder (determined at NOTOX)
Batch 259-635
Purity > 90%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Frequency at t=0 h and t=48 h
Volume 2 ml from the approximate centre of the test vessels
Storage Samples were stored in a freezer until analysis.

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Hardness:
180 mg/l as CaCO3
Test temperature:
19.7 - 20.8°C
pH:
7.8
Dissolved oxygen:
8.9 - 9.8
Nominal and measured concentrations:
Analysis of the samples taken from the filtrates prepared at 1.0 and 100 mg/l showed that measured concentrations remained fairly stable over the test period. Analyses of the filtrate prepared at 1.0 mg/l showed measured concentrations of 11.0 and 12.1 µg/l at t=0 and 48 hours. Analyses of the filtrate prepared at 100 mg/l showed measured concentrations of 17.1 and 19.6 µg/l at t=0 and 48 hours, respectively. The average measured concentrations were calculated to correspond to 12 and 18 µg/l, for the filtrates prepared at 1.0 and 100 mg/l, respectively.
Details on test conditions:
Test vessels 100 ml, all-glass
Medium Adjusted ISO medium
Number of daphnids 20 per concentration
Loading 5 per vessel containing 80 ml of test solution
Light 16 hours photoperiod daily
Feeding No feeding
Aeration No aeration of the test solutions.
Introduction of daphnids Within 1 hour after preparation of the test solutions.

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The 48h-EC50 exceeded 18 µg/l, being the average measured concentration in a 0.45 µm filtered solution prepared at a loading rate of 100 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes