2-propylheptan-1-ol

Toxicity to soil microorganisms

Administrative data

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011-02-23 to 2011-09-10

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-propylheptan-1-ol

EC Number:

233-126-1

EC Name:

2-propylheptan-1-ol

Cas Number:

10042-59-8

Molecular formula:

C10H22O

IUPAC Name:

2-propylheptan-1-ol

Details on test material:

- Physical state: Clear and colorless liquid
- Analytical purity: 94.7 area-%
- Lot/batch No.: Tk2012_20110113
- Expiry date: 13 Jan 2012
- Storage condition of test material: At room temperature

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Sampling procedure for determination of formed nitrate in the test assays: Three samples from of each test assay were taken at the start of exposure, after 7 days and after 28 days for determination the content of nitrate. Therefore the amount of about 23.5 g was weighed into glass bottles and 100 mL demineralized water were added to aliquots of the test assays. The soil suspensions were shaken for about 1 hour at 150 rpm with a
laboratory shaker. Volumes of about 10 mL of the suspensions were taken and centrifuged for about 30 minutes at 4500 rpm. The content of formed nitrate was measured as nitrate nitrogen in each supernatant using an analytical kit no. LCK 339 from. Hach-Lange. The nitrate values of the test assays with testsubstance were compared with the values of formed nitrate of the blank control.

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

AMENDMENT OF SOIL
- Type of organic substrate: Natural soil, type 2.3

APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: The required amount of the test substance for each test concentration were balanced with 5 g quartz sand as carrier (equal to 10 g/kg dry weight) and mixed well.

Test organisms

Test organisms (inoculum):

soil

Study design

Total exposure duration:

28 d

Test conditions

Test temperature:

20 ± 2°C

Moisture:

Water content of the soil (measured): 5.5 g/100g dry weight
Water content of the test mixture (soil, test substance and quartz sand): 16 g/100 g dw
Water content of the soil during exposure: 45 ± 5% of the WHCmax

Details on test conditions:

TEST SYSTEM
- Testing facility: BASF SE, Ludwigshafen, Germany
- Amount of soil: ca. 581 g
- No. of replicates per concentration: 2
- No. of replicates per control: 2

SOIL INCUBATION
- Method: Series of individual subsamples

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Source of substrate: Landwirtschaftliche Untersuchungs- und Forschungsanstalt Speyer, Obere Langgasse 40, 67346 Speyer, Germany
- Geographical reference of sampling site: Im Bildgarten, Nr 508/2
- Depth of sampling: Approx. 20 cm
- Soil taxonomic classification: Silty sand according to German DIN
- pH: 6.7 ± 0.3
- Treatment with pesticides or fertilizers: The soil was not treated with crop protection products for > 4 years. The sampling field was not cultivated for 5 years and no fertilizer application was performed during the last 5 years before sampling the soil.
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): 93 to 110
- Cation exchange capacity: 10.0 ± 2.0 mval/100 g
- Pretreatment of soil: Drying for 6 days at room temperature, Presieving with 10 mm screen, Last sieving 2 mm screen, water content 4.5 g/100 g dw, at the day of exposure the soil was mixed with 16.6 g luzerne meal.
- Initial microbial biomass: 107.8 ± 0.1 mg carbon/kg dw/h

EFFECT PARAMETERS MEASURED: Nitrogen transformation after 7 and after 28 days.

Nominal and measured concentrations:

Nominal concentrations: 62.5, 125, 250, 500 and 1000 mg/kg dw

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

7 d

Dose descriptor:

EC10

Effect conc.:

28 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Remarks on result:

other: 95% CL: 5.3 – 153 mg/kg dw

Effect concentrations 2

Duration:

7 d

Dose descriptor:

EC25

Effect conc.:

194 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Remarks on result:

other: 95% CL: 98– 384 mg/kg dw

Effect concentrations 3

Duration:

7 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Remarks on result:

other: 95% CL: 524 - 5090 mg/kg dw

Effect concentrations 4

Duration:

28 d

Dose descriptor:

EC10

Effect conc.:

341 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Remarks on result:

other: 95% CL: 293 – 396 mg/kg dw

Effect concentrations 5

Duration:

28 d

Dose descriptor:

EC25

Effect conc.:

561 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Remarks on result:

other: 95% CL: 516– 610 mg/kg dw

Effect concentrations 6

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

976 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Remarks on result:

other: 95% CL: 901 - 1057 mg/kg dw